ArticleVirologica Sinica2022
A single nonsynonymous mutation on ZIKV E protein-coding sequences leads to markedly increased neurovirulence in vivo.
Article in Virologica Sinica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 18 citations in OpenAlex.
- Sequence-Dependent PNA-AgNP Bioconjugation Enables Electrochemical Discrimination of a Single-Base Mutation in Zika Virus RNA.ACS omega · 2026Article
- Extraction, structural characterization and SNP identification of related genes of collagen from local pigs in Guizhou.BMC genomics · 2026Article
- Breaking barriers and beyond: Mechanisms and pathological implications of Zika virus traversal across blood-tissue interfaces.Virologica Sinica · 2026Review
- Impact of Selenium and Vitamin E Deficiency on Zika Virus Pathogenesis and Immune Response in Mice.Viruses · 2026Article
- Tembusu virus neuroinvasion and neuropathogenesis: lessons from blood-brain barrier dysfunction in neurotropic flaviviruses.Frontiers in veterinary science · 2026Review
- An Emerging DTMUV Variant Remains Restricted in Mammals but Acquires Olfactory Route-Mediated Neuroinvasion Following Limited Adaptive Mutations.Transboundary and emerging diseases · 2026Article
- Evolution of Zika virus inVirus evolution · 2025Article
- Epidemic Zika virus strains from the Asian lineage induce an attenuated fetal brain pathogenicity.Nature communications · 2024Article
- An accumulated mutation gained in mosquito cells enhances Zika virus virulence and fitness in mice.Journal of virology · 2024Article
- Fitness adaptations of Japanese encephalitis virus in pigs following vector-free serial passaging.PLoS pathogens · 2024Article
- CRISPR/Cas9-Mediated Knockout of theMicroorganisms · 2024Article
- Genomic variability in Zika virus in GBS cases in Colombia.PloS one · 2024Article
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- An overview of Zika virus genotypes and their infectivity.Revista da Sociedade Brasileira de Medicina Tropical · 2022Review
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Authors and funding
11 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zika virus (ZIKV) can infect a wide range of tissues including the developmental brain of human fetus. Whether specific viral genetic variants are linked to neuropathology is incompletely understood. To address this, we have intracranially serially passaged a clinical ZIKV isolate (SW01) in neonatal mice and discovered variants that exhibit markedly increased virulence and neurotropism. Deep sequencing analysis combining with molecular virology studies revealed that a single 67D (Aspartic acid) to N (Asparagine) substitution on E protein is sufficient to confer the increased virulence and neurotropism in vivo. Notably, virus clones with D67N mutation had higher viral production and caused more severe cytopathic effect (CPE) in human neural astrocytes U251 cells in vitro, indicating its potential neurological toxicity to human brain. These findings revealed that a single mutation D67N on ZIKV envelope may lead to severe neuro lesion that may help to explain the neurovirulence of ZIKV and suggest monitoring the occurrence of this mutation during nature infection may be important.
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