Evidence map›Paper›PMID 35234510›Full record

ArticleMicrobiology spectrum2022

HopE and HopD Porin-Mediated Drug Influx Contributes to Intrinsic Antimicrobial Susceptibility and Inhibits Streptomycin Resistance Acquisition by Natural Transformation in Helicobacter pylori.

Yixin Liu, Feng Yang, Su Wang, Wenjing Chi, Li Ding, Tao Liu, Feng Zhu, Danian Ji, Jun Zhou, Yi Fang and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Asclepain cI, a proteolytic enzyme fromFrontiers in microbiology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 1 institution in 1 country.

Yixin LiuDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Feng YangDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.ORCID 0000-0001-6031-9038
Su WangDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Wenjing ChiDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Li DingDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Tao LiuDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Feng ZhuDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Danian JiDepartment of Endoscopy, Huadong Hospital, Fudan University, Shanghai, China.
Jun ZhouDepartment of Endoscopy, Huadong Hospital, Fudan University, Shanghai, China.
Yi FangDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Jinghao ZhangDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Ping XiangDepartment of Endoscopy, Huadong Hospital, Fudan University, Shanghai, China.
Yanmei ZhangDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Hu ZhaoDepartment of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China.
Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Helicobacter pylori is a human pathogen competent for natural transformation. Intrinsic and acquired antibiotic resistance contribute to the survival and multiplication of H. pylori under antibiotics. While drug-resistance dissemination by natural transformation (NT)-mediated horizontal gene transfer remains poorly understood in H. pylori. The purpose of the study was to investigate the role of H. pylori porins (HopA, HopB, HopC, HopD, and HopE) in the intrinsic antibiotic resistance and to preliminarily reveal the potential effect of HopE and HopD porins in streptomycin resistance acquisition after NT in the presence of antibiotics. Using traditional antibiotic susceptibility tests and growth curve analysis, we found the MIC values of metronidazole, clarithromycin, levofloxacin, tetracycline, rifampin, and streptomycin in mutants lacking HopE and/or HopD were significantly elevated compare to those in wild-type strain. The quantitative analysis of the tetramethyl rhodamine isothiocyanate (TRITC)-labeled streptomycin accumulation at the single-cell level showed reduced streptomycin intracellular fluorescence in Δ

Indexed as

Anti-Infective AgentsHelicobacter InfectionsHelicobacter pyloriAnti-Bacterial AgentsDrug Resistance, BacterialHumansMicrobial Sensitivity TestsPorinsStreptomycinAnti-Bacterial AgentsAnti-Infective AgentsPorinsStreptomycinantibiotic resistanceHelicobacter pyloriHopDHopEH. pylorinatural transformationporin

Identifiers

PMID35234510
PMCPMC9045298
OpenAlexW4214919420

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.