ArticleThe Plant cell2022
A phytoplasma effector acts as a ubiquitin-like mediator between floral MADS-box proteins and proteasome shuttle proteins.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Phytoplasma-plant interactions: effector-mediated host reprogramming, hormonal crosstalk, metabolic alterations and plant-mediated vector manipulation.World journal of microbiology & biotechnology · 2026Review
- The pathogenic effector PHYL1The Plant journal : for cell and molecular biology · 2026Article
- A Virus-Inducible E3-RLCK-MADS Module Coordinates Suppression of Plant Immunity and Fertility in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Tomato ubiquitinome in response to 'Candidatus Liberibacter solanacearum' haplotypes A and B.Crop health · 2026Article
- Roles of MADS-box transcription factors in plant responses to abiotic and biotic stresses.Plant communications · 2026Review
- Molecular Mechanism of ZjWRKY40-zju-miR157 Module Regulating Phytoplasma Tolerance in Jujube.Molecular plant pathology · 2026Article
- Extended phenotype of phytoplasmas in eukaryotic systems: mechanisms and ecological implications.Frontiers in plant science · 2026Review
- Effector innovation in genome-reduced phytoplasmas and other host-dependent mollicutes.PLoS genetics · 2025Article
- The sucrose signalling route controls Flavescence dorée phytoplasma load in grapevine leaves.Journal of experimental botany · 2025Article
- Discovery of two tightly linked soybean genes at the qSCN10 (O) locus conferring broad-spectrum resistance to soybean cyst nematode.Communications biology · 2025Article
- Ubiquitin-Independent Degradation: An Emerging PROTAC Approach?BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- A phytoplasma effector suppresses insect melanization immune response to promote pathogen persistent transmission.Science advances · 2025Article
- Article
- A phytoplasma effector destabilizes chloroplastic glutamine synthetase inducing chlorotic leaves that attract leafhopper vectors.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structure basis for recognition of plant Rpn10 by phytoplasma SAP05 in ubiquitin-independent protein degradation.iScience · 2024Article
- Phytoplasma: A plant pathogen that cannot be ignored in agricultural production-Research progress and outlook.Molecular plant pathology · 2024Review
- Target degradation specificity of phytoplasma effector phyllogen is regulated by the recruitment of host proteasome shuttle protein.Molecular plant pathology · 2024Article
- Bimodular architecture of bacterial effector SAP05 that drives ubiquitin-independent targeted protein degradation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Random mutagenesis-based screening of the interface of phyllogen, a bacterial phyllody-inducing effector, for interaction with plant MADS-box proteins.Frontiers in plant science · 2023Article
- Potential mobile units drive the horizontal transfer of phytoplasma effectorFrontiers in genetics · 2023Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant pathogenic bacteria have developed effectors to manipulate host cell functions to facilitate infection. A certain number of effectors use the conserved ubiquitin-proteasome system in eukaryotic to proteolyze targets. The proteasome utilization mechanism is mainly mediated by ubiquitin interaction with target proteins destined for degradation. Phyllogens are a family of protein effectors produced by pathogenic phytoplasmas that transform flowers into leaves in diverse plants. Here, we present a noncanonical mechanism for phyllogen action that involves the proteasome and is ubiquitin-independent. Phyllogens induce proteasomal degradation of floral MADS-box transcription factors (MTFs) in the presence of RADIATION-SENSITIVE23 (RAD23) shuttle proteins, which recruit ubiquitinated proteins to the proteasome. Intracellular localization analysis revealed that phyllogen induced colocalization of MTF with RAD23. The MTF/phyllogen/RAD23 ternary protein complex was detected not only in planta but also in vitro in the absence of ubiquitin, showing that phyllogen directly mediates interaction between MTF and RAD23. A Lys-less nonubiquitinated phyllogen mutant induced degradation of MTF or a Lys-less mutant of MTF. Furthermore, the method of sequential formation of the MTF/phyllogen/RAD23 protein complex was elucidated, first by MTF/phyllogen interaction and then RAD23 recruitment. Phyllogen recognized both the evolutionarily conserved tetramerization region of MTF and the ubiquitin-associated domain of RAD23. Our findings indicate that phyllogen functionally mimics ubiquitin as a mediator between MTF and RAD23.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.