Evidence map›Paper›PMID 35234168›Full record

ArticleJournal of musculoskeletal & neuronal interactions2022

miR-205-5p in exosomes divided from chondrogenic mesenchymal stem cells alleviated rheumatoid arthritis via regulating MDM2 in fibroblast-like synoviocytes.

Wukai Ma, Fang Tang, Lina Xiao, Shan Han, Xueming Yao, Qiongyu Zhang, Jiayan Zhou, Yanjun Wang, Jing Zhou

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Journal of musculoskeletal & neuronal interactions, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Review
  15. Article
  16. Review
  17. Review
  18. Role of Exosomal miR-205-5p Cargo in Angiogenesis and Cell Migration.International journal of molecular sciences · 2024
    Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Wukai Ma *Rheumatology and Immunology Department, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou, China.
Fang Tang *Rheumatology and Immunology Department, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou, China.
Lina Xiao *Nursing Department, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou, China.
Shan HanGraduate School, Guizhou University of Traditional Chinese Medicine, Guizhou, China.
Xueming YaoRheumatology and Immunology Department, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou, China.
Qiongyu ZhangRheumatology and Immunology Department, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou, China.
Jiayan ZhouRheumatology and Immunology Department, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou, China.
Yanjun WangGraduate School, Guizhou University of Traditional Chinese Medicine, Guizhou, China.
Jing ZhouRheumatology and Immunology Department, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou, China.
Affiliated Hospital of Guizhou Medical University · CNGuizhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo explore the role and mechanism of chondrogenic bone marrow mesenchymal stem cells (BMSCs)-derived exosomes on Rheumatoid arthritis (RA).

methodsThe chondrogenesis of BMSCs was induced by chondrogenic medium. Exosomes from BMSCs and chondrogenic BMSCs were isolated and characterized by transmission electron microscope (TEM), laser particle size analyzer and western blot. ELISA was used to analyze the expression levels of pro-inflammatory cytokines and matrix metalloproteinases (MMPs). Western bolt was performed to assess MAPK and NF-κB pathways expression. The inflammation score and the pathological damage of RA mice were evaluated. Luciferase reporter assay and RIP were carried out to examine the relationship between microRNA-205-5p (miR-205-5p) and mouse double minute 2 (MDM2).

resultsChondrogenic BMSCs-derived exosomes suppressed pro-inflammatory cytokines, MMPs and MAPK and NF-κB pathways in RA-FLSs. miR-205-5p had a high expression in chondrogenic BMSCs-derived exosomes. Functionally, exosomal miR-205-5p also played the anti-inflammation effects. Besides, MDM2 was a direct target of miR-205-5p. Additionally, chondrogenic BMSCs-secreted exosomal miR-205-5p suppressed the inflammation score, joint destruction, and inflammatory response in collagen-induced arthritis (CIA) mice through MDM2.

conclusionChondrogenic BMSCs-derived exosomal miR-205-5p suppressed inflammatory response, MAPK and NF-κB pathways through MDM2 in RA, indicating exosomal miR-205-5p might be a potential target for RA treatment.

Indexed as

Arthritis, RheumatoidExosomesMesenchymal Stem CellsMicroRNAsProto-Oncogene Proteins c-mdm2SynoviocytesAnimalsChondrogenesisCytokinesFibroblastsInflammationMiceNF-kappa BCytokinesMdm2 protein, mouseMicroRNAsMIRN205 microRNA, mouseNF-kappa BProto-Oncogene Proteins c-mdm2BMSCsExosomesMDM2miR-205-5pRheumatoid Αrthritis

Identifiers

PMID35234168
PMCPMC8919649
OpenAlexW4214955376

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.