ArticleScientific reports2022
Transcriptome profiling revealed candidate genes, pathways and transcription factors related to nitrogen utilization and excessive nitrogen stress in perennial ryegrass.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 20 citations in OpenAlex.
- Variations in methane emissions from dairy cows: associations with rumen microbial synergy and metabolic pathway divergence.Journal of animal science and biotechnology · 2026Article
- Combined transcriptome and metabolome analysis reveals nitrogen-responsive genes and metabolites in sugar beet (Frontiers in plant science · 2026Article
- Genome-wide identification and expression patterns analysis of the DREB gene family under various abiotic stress in Lolium perenne.BMC plant biology · 2025Article
- Joint multi-omics analysis reveals the response mechanism in rapeseed (Brassica Rapa L.) under low nitrogen stress.Functional & integrative genomics · 2025Article
- Genome-wide analysis of the AP2/ERF transcription factor superfamily in perennial ryegrass.BMC genomics · 2025Article
- Morphological and Transcriptomic Analyses Provide New Insights into Linseed (Plants (Basel, Switzerland) · 2025Article
- Integrated transcriptomic and metabolomic analysis of flavonoid biosynthesis in cigar tobacco leaves under variable nitrogen regimes.Frontiers in plant science · 2025Article
- Biomass enhancement and activation of transcriptional regulation in sorghum seedling by plasma-activated water.Frontiers in plant science · 2024Article
- JGI Plant Gene Atlas: an updateable transcriptome resource to improve functional gene descriptions across the plant kingdom.Nucleic acids research · 2023Article
- Chromosome-level genome and high nitrogen stress response of the widespread and ecologically important wetland plantFrontiers in plant science · 2023Article
- Physiology and transcriptome analysis of the response mechanism ofFrontiers in plant science · 2023Article
- Transcriptional regulation mechanism of wheat varieties with different nitrogen use efficiencies in response to nitrogen deficiency stress.BMC genomics · 2022Article
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7 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Ryegrass (Lolium perenne L.), a high-quality forage grass, is a good nutrient source for herbivorous livestock. However, improving nitrogen use efficiency and avoiding nitrate toxicity caused by excessive nitrogen are continual challenges in ryegrass production. The molecular mechanism underlying the response of ryegrass to nitrogen, especially excessive nitrogen, remains unclear. In this study, the transcriptomic changes under different nitrogen levels were investigated in perennial ryegrass by high-throughput next-generation RNA sequencing. Phenotypic characterization showed that treatment with half of the standard N concentration (N0.5) led to a better growth state than the other three treatments. The treatments with the standard N concentration (N1) and treatments with ten times higher than the standard N concentration (N10) contained excessive nitrogen, which placed stress on plant growth. Analysis of differentially expressed genes indicated that 345 and 104 genes are involved in the regulation of nitrogen utilization and excessive nitrogen stress, respectively. KEGG enrichment analysis suggested that "photosynthesis-antenna proteins" may respond positively to appropriate nitrogen conditions, whereas "steroid biosynthesis", "carotenoid biosynthesis" and "C5-branched dibasic acid metabolism" were identified as the top significantly enriched pathways in response to excessive nitrogen. Additionally, 21 transcription factors (TFs) related to nitrogen utilization were classified into 10 families, especially the AP2-EREBP and MYB TF families. Four TFs related to excessive nitrogen stress were identified, including LOBs, NACs, AP2-EREBPs and HBs. The expression patterns of these selected genes were also analyzed. These results provide new insight into the regulatory mechanism of ryegrass in response to nitrogen utilization and excessive nitrogen stress.
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