ArticleBMC genomics2022
Characterization of UDP-glycosyltransferase family members reveals how major flavonoid glycoside accumulates in the roots of Scutellaria baicalensis.
Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 37 citations in OpenAlex.
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- Potential new drug sources for the treatment of Parkinson's disease: flavonoid O-glycosides.Molecular biology reports · 2025Review
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- Integrated Metabolome, Transcriptome, and Physiological Analysis of the Flavonoid and Phenylethanol Glycosides Accumulation in WildInternational journal of molecular sciences · 2025Article
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- Strain-Specific Infection of Phage AP1 to Rice Bacterial Brown Stripe PathogenPlants (Basel, Switzerland) · 2024Article
- Computational-guided discovery of UDP-glycosyltransferases for lauryl glucoside production using engineered E. coli.Bioresources and bioprocessing · 2024Article
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- The Chrysosplenium sinicum genome provides insights into adaptive evolution of shade plants.Communications biology · 2024Article
- Multi-omics landscape to decrypt the distinct flavonoid biosynthesis ofHorticulture research · 2024Article
- Gap-free genome assembly andHorticulture research · 2023Article
- Identification for metabolism profiles and pharmacokinetic studies of tradition Chinese prescription Ji-Ming-San and its major metabolites in rats by UHPLC-Q-TOF-MS/MS and UHPLC-MS/MS.Journal of food and drug analysis · 2023Article
- Plant Secondary Metabolites: The Weapons for Biotic Stress Management.Metabolites · 2023Review
- SbMYB3 transcription factor promotes root-specific flavone biosynthesis inHorticulture research · 2023Article
- The chemical profiling ofFrontiers in plant science · 2023Article
- Genome-wide analysis ofSynthetic and systems biotechnology · 2022Article
- Biosynthesis Investigations of Terpenoid, Alkaloid, and Flavonoid Antimicrobial Agents Derived from Medicinal Plants.Antibiotics (Basel, Switzerland) · 2022Review
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11 authors at 3 institutions in 1 country.
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Abstract
backgroundFlavonoid glycosides extracted from roots of Scutellaria baicalensis exhibit strong pharmaceutical antitumor, antioxidative, anti-inflammatory, and antiviral activities. UDP glycosyltransferase (UGT) family members are responsible for the transfer of a glycosyl moiety from UDP sugars to a wide range of acceptor flavonoids. Baicalin is the major flavonoid glycoside found in S. baicalensis roots, and its aglycone baicalein is synthesized from a specially evolved pathway that has been elucidated. However, it is necessary to carry out a genome-wide study of genes involved in 7-O-glucuronidation, the final biosynthesis step of baicalin, which might elucidate the relationship between the enzymes and the metabolic accumulation patterns in this medicinal plant.
resultsWe reported the phylogenetic analysis, tissue-specific expression, biochemical characterization and evolutionary analysis of glucosyltransferases (SbUGTs) and glucuronosyltransferases (SbUGATs) genes based on the recently released genome of S. baicalensis. A total of 124 UGTs were identified, and over one third of them were highly expressed in roots. In vitro enzyme assays showed that 6 SbUGTs could use UDP-glucose as a sugar donor and convert baicalein to oroxin A (baicalein 7-O-glucoside), while 4 SbUGATs used only UDP-glucuronic acid as the sugar donor and catalyzed baicalein to baicalin. SbUGAT4 and SbUGT2 are the most highly expressed SbUGAT and SbUGT genes in root tissues, respectively. Kinetic measurements revealed that SbUGAT4 had a lower Km value and higher Vmax/Km ratio to baicalein than those of SbUGT2. Furthermore, tandem duplication events were detected in SbUGTs and SbUGATs.
conclusionsThis study demonstrated that glucosylation and glucuronidation are two major glycosylated decorations in the roots of S. baicalensis. Higher expression level and affinity to substrate of SbUGAT4, and expansion of this gene family contribute high accumulation of baicalin in the root of S. baicalensis.
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