Evidence map›Paper›PMID 35231074›Full record

ArticlePloS one2022

Cistanche promotes the adipogenesis of 3T3-L1 preadipocytes.

Ping Zhang, Le Su, Xiuyu Ji, Feifan Ma, Qiulin Yue, Chen Zhao, Song Zhang, Xin Sun, Kunlun Li, Lin Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ping ZhangState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China.
Le SuState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China.
Xiuyu JiState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China.
Feifan MaState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China.
Qiulin YueState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China.
Chen ZhaoShandong Provincial Key Laboratory of Food and Fermentation Engineering, Shandong Food Ferment Industry Research & Design Institute, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China.
Song ZhangState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China.
Xin SunState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China.
Kunlun LiJinan Hang Chen Biotechnology Co., Ltd., Jinan, China.
Lin ZhaoState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, China.ORCID 0000-0002-4016-3176

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cistanche deserticola Ma (cistanche) is a traditional herb with a wide range of therapeutic properties. However, no evidence of cistanche's effect on adipogenesis has been found. The effect of cistanche that promotes the adipogenesis of 3T3-L1 preadipocytes was proved by using MTT spectrophotometry, Nile Red staining, Oil Red O staining and transcriptome sequencing technology. The mRNA level of key transcription factors for adipogenesis such as PPAR, AP2 and LPL were examined by RT-PCR. The results showed that the intracellular lipid content in cistanche treated cells were notably increased when compared with the non-treated cells. Between the differentiation and cistanche treated groups, the expression of adipogenesis related genes such as grow hormone releasing hormone (Ghrp), BCL2/adenovirus E1B interacting protein 3 (Bnip3) and Gastric inhibitory polypeptide receptor (Gipr) were significantly increased. Our findings also verified that cistanche promoted adipogenesis, which was accompanied by up-regulated level of Bnip3 and PPAR. This study could uncover new signaling pathways involved in adipogenesis regulation.

Indexed as

AdipogenesisCistanche3T3-L1 CellsAdipocytesAnimalsCell DifferentiationHormonesMicePPAR gammaHormonesPPAR gamma

Identifiers

PMID35231074
PMCPMC8887766

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.