Evidence map›Paper›PMID 35229169›Full record

ArticleHistochemistry and cell biology2022

Angiotensin II type 1 receptor localizes at the blood-bile barrier in humans and pigs.

Galyna Pryymachuk, Ehab El-Awaad, Nadin Piekarek, Uta Drebber, Alexandra C Maul, Juergen Hescheler, Andreas Wodarz, Gabriele Pfitzer, Wolfram F Neiss, Markus Pietsch and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Histochemistry and cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. In focus in HCB.Histochemistry and cell biology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 2 countries.

Galyna PryymachukDepartment of Anatomy I, University of Cologne, Faculty of Medicine and University Hospital Cologne, Kerpener Str. 62, 50937, Cologne, Germany. galyna.pryymachuk@uk-koeln.de.ORCID http://orcid.org/0000-0002-6971-2802
Ehab El-AwaadInstitute II of Pharmacology, Center of Pharmacology, University of Cologne, Faculty of Medicine and University Hospital Cologne, Gleueler Str. 24, 50931, Cologne, Germany.ORCID http://orcid.org/0000-0003-3383-6384
Nadin PiekarekDepartment of Anatomy I, University of Cologne, Faculty of Medicine and University Hospital Cologne, Kerpener Str. 62, 50937, Cologne, Germany.ORCID http://orcid.org/0000-0002-2954-0145
Uta DrebberInstitute of Pathology, University of Cologne, Faculty of Medicine and University Hospital Cologne, Kerpener Str. 62, 50937, Cologne, Germany.ORCID http://orcid.org/0000-0002-2697-4685
Alexandra C MaulExperimental Medicine, University of Cologne, Faculty of Medicine and University Hospital Cologne, Ostmerheimer Str. 200, 51109, Cologne, Germany.ORCID http://orcid.org/0000-0003-0621-0968
Juergen HeschelerInstitute for Neurophysiology, Center for Physiology and Pathophysiology, University of Cologne, Faculty of Medicine and University Hospital Cologne, Robert-Koch-Str. 39, 50931, Cologne, Germany.ORCID http://orcid.org/0000-0001-7686-6562
Andreas WodarzDepartment of Anatomy I, University of Cologne, Faculty of Medicine and University Hospital Cologne, Kerpener Str. 62, 50937, Cologne, Germany.ORCID http://orcid.org/0000-0002-0007-6289
Gabriele PfitzerInstitute of Vegetative Physiology, Center for Physiology and Pathophysiology, University of Cologne, Faculty of Medicine and University Hospital Cologne, Robert-Koch-Str. 39, 50931, Cologne, Germany.ORCID http://orcid.org/0000-0002-8684-8947
Wolfram F NeissDepartment of Anatomy I, University of Cologne, Faculty of Medicine and University Hospital Cologne, Kerpener Str. 62, 50937, Cologne, Germany.ORCID http://orcid.org/0000-0002-5035-5825
Markus PietschInstitute II of Pharmacology, Center of Pharmacology, University of Cologne, Faculty of Medicine and University Hospital Cologne, Gleueler Str. 24, 50931, Cologne, Germany.ORCID http://orcid.org/0000-0002-8110-2265
Mechthild M SchroeterInstitute for Neurophysiology, Center for Physiology and Pathophysiology, University of Cologne, Faculty of Medicine and University Hospital Cologne, Robert-Koch-Str. 39, 50931, Cologne, Germany.ORCID http://orcid.org/0000-0003-0184-1146
University of Cologne · DE

Funding

Deutsche Forschungsgemeinschaft INST 1856/66-1 FUGGGerman Academic Exchange Service-German Egyptian Research Long-Term scholarship 91541390German Academic Exchange Service-German Egyptian Research Long-Term scholarship A/12/93239Graduate Program in Pharmacology and Experimental Therapeutics of the University of Cologne and Bayer Healthcare N14
6 · The paper itself

Abstract

Animal models and clinical studies suggest an influence of angiotensin II (AngII) on the pathogenesis of liver diseases via the renin-angiotensin system. AngII application increases portal blood pressure, reduces bile flow, and increases permeability of liver tight junctions. Establishing the subcellular localization of angiotensin II receptor type 1 (AT1R), the main AngII receptor, helps to understand the effects of AngII on the liver. We localized AT1R in situ in human and porcine liver and porcine gallbladder by immunohistochemistry. In order to do so, we characterized commercial anti-AT1R antibodies regarding their capability to recognize heterologous human AT1R in immunocytochemistry and on western blots, and to detect AT1R using overlap studies and AT1R-specific blocking peptides. In hepatocytes and canals of Hering, AT1R displayed a tram-track-like distribution, while in cholangiocytes AT1R appeared in a honeycomb-like pattern; i.e., in liver epithelia, AT1R showed an equivalent distribution to that in the apical junctional network, which seals bile canaliculi and bile ducts along the blood-bile barrier. In intrahepatic blood vessels, AT1R was most prominent in the tunica media. We confirmed AT1R localization in situ to the plasma membrane domain, particularly between tight and adherens junctions in both human and porcine hepatocytes, cholangiocytes, and gallbladder epithelial cells using different anti-AT1R antibodies. Localization of AT1R at the junctional complex could explain previously reported AngII effects and predestines AT1R as a transmitter of tight junction permeability.

Indexed as

BileReceptor, Angiotensin, Type 1Angiotensin IIAnimalsBlotting, WesternHumansPeptidesReceptor, Angiotensin, Type 2SwineAngiotensin IIPeptidesReceptor, Angiotensin, Type 1Receptor, Angiotensin, Type 2AT1RCanals of HeringGallbladderHuman liverPorcine liverTight junctions

Identifiers

PMID35229169
PMCPMC9114028
OpenAlexW4214849174

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.