Evidence map›Paper›PMID 35227325›Full record

ArticleInflammation and regeneration2022

Generation of a BAC transgenic mouse strain that expresses CreERT and a fluorescent protein under the transcriptional control of the Fzd5 locus.

Satoru Miyagi, Yuko Kato, Ayako Watanabe, Kenichi Miyamoto, Rintaro Yoshikawa, Keita Hagiya, Daisuke Hirano, Yumi Matsuzaki

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Inflammation and regeneration, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Satoru Miyagi *Department of Life Science, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan. miyagi@med.shimane-u.ac.jp.
Yuko Kato *Department of Life Science, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
Ayako WatanabeDepartment of Life Science, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
Kenichi MiyamotoDepartment of Life Science, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
Rintaro YoshikawaDepartment of Life Science, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
Keita HagiyaFUJIFILM Corporation, Minato-ku, Tokyo, Japan.
Daisuke HiranoFUJIFILM Corporation, Minato-ku, Tokyo, Japan.
Yumi MatsuzakiDepartment of Life Science, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan. matsuzak@med.shimane-u.ac.jp.
Shimane University · JPFujifilm (Japan) · JP

Funding

Ministry of Education, Culture, Sports, Science and Technology, Japan #19K08865
6 · The paper itself

Abstract

backgroundThe expression of FZD5 distinguishes immature human mesenchymal stem/stromal cells (MSC) in cultures, and the function of FZD5 is crucial for maintaining the proliferation and multilineage differentiation capacity of human MSC. We herein investigated whether Fzd5 expression also marks undifferentiated MSC in animals.

methodsWe generated a transgenic mouse strain (Fzd5-CreERT-tFP635) that expresses CreERT and the fluorescent protein, TurboFP635 (tFP635), under the transcriptional control of the Fzd5 gene using the BAC transgenic technique, and identified cells expressing tFP635 by flow cytometry. We also conducted lineage tracing with this strain.

resultsIn the bone marrow of transgenic mice, tFP635 was preferentially expressed in MSC, Leptin receptor-expressing MSC (LepR

conclusionsOur transgenic mouse marks two different types of MSC, LepR

Indexed as

Fzd5Leptin receptor-expressing MSC (LepR+MSC)Mesenchymal stem/stromal cells (MSC)PαS

Identifiers

PMID35227325
PMCPMC8886790
OpenAlexW4214674687

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.