ArticleJournal of visualized experiments : JoVE2022
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots.
Article in Journal of visualized experiments : JoVE, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 15 citations in OpenAlex.
- Label-free, non-optical detection of intact SARS-CoV-2 virions in native human saliva via microscale surface ion conduction.Biosensors & bioelectronics · 2026Article
- Cellular Metabolic Signatures of Long COVID-19.Infectious disease reports · 2026Article
- Surrogate virus neutralization test to determine salivary neutralizing antibody inhibition of ACE2 and SARS-CoV-2 spike RBD complex.Frontiers in immunology · 2026Article
- Development and validation of automated methods for COVID-19 PCR Master Mix preparation.SLAS technology · 2024Article
- SARS-CoV-2 variant introduction following spring break travel and transmission mitigation strategies.PloS one · 2024Article
- Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle.Trends in analytical chemistry : TRAC · 2023Review
- Pooled RNA-extraction-free testing of saliva for the detection of SARS-CoV-2.Scientific reports · 2023Article
- A capacitive laser-induced graphene based aptasensor for SARS-CoV-2 detection in human saliva.PloS one · 2023Article
- Repurposing a SARS-CoV-2 surveillance program for infectious respiratory diseases in a university setting.Frontiers in public health · 2023Article
- Predicting COVID-19 Infected Individuals in a Defined Population from Wastewater RNA Data.ACS ES&T water · 2022Article
- Identifying SARS-CoV-2 Variants of Concern through Saliva-Based RT-qPCR by Targeting Recurrent Mutation Sites.Microbiology spectrum · 2022Article
- SARS-CoV-2 variants of concern Alpha and Delta show increased viral load in saliva.medRxiv : the preprint server for health sciences · 2022Article
- Identifying SARS-CoV-2 Variants of Concern through saliva-based RT-qPCR by targeting recurrent mutation sites.medRxiv : the preprint server for health sciences · 2022Article
- Implementation of a Rural Community Diagnostic Testing Strategy for SARS-CoV-2 in Upstate South Carolina.Frontiers in public health · 2022Article
- Development of a Biosensor Based on Angiotensin-Converting Enzyme II for Severe Acute Respiratory Syndrome Coronavirus 2 Detection in Human Saliva.Frontiers in sensors · 2022Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The emergence of the recent SARS-CoV-2 global health crisis introduced key challenges for epidemiological research and clinical testing. Characterized by a high rate of transmission and low mortality, the COVID-19 pandemic necessitated accurate and efficient diagnostic testing, particularly in closed populations such as residential universities. Initial availability of nucleic acid testing, like nasopharyngeal swabs, was limited due to supply chain pressure which also delayed reporting of test results. Saliva-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) testing has shown to be comparable in sensitivity and specificity to other testing methods, and saliva collection is less physically invasive to participants. Consequently, we developed a multiplex RT-qPCR diagnostic assay for population surveillance of Clemson University and the surrounding community. The assay utilized open-source liquid handling robots and thermocyclers instead of complex clinical automation systems to optimize workflow and system flexibility. Automation of saliva-based RT-qPCR enables rapid and accurate detection of a wide range of viral RNA concentrations for both large- and small-scale testing demands. The average turnaround for the automated system was < 9 h for 95% of samples and < 24 h for 99% of samples. The cost for a single test was $2.80 when all reagents were purchased in bulk quantities.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.