ArticleDevelopment (Cambridge, England)2022
Single-cell RNA sequencing reveals PDGFRα+ stromal cell subpopulations that promote proacinar cell differentiation in embryonic salivary gland organoids.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Fostering cell-cell interactions and integrating angiocrine factors to promote the development of salivary microtissues in 3D.Bioengineering & translational medicine · 2026Article
- A Mouthful of Genomic Data: Single-Cell Insights into Salivary Gland Biology and Disease.Biology · 2026Review
- Functional human salivary gland organoids for tissue regeneration in chemically defined culture systems.Cell reports. Medicine · 2026Article
- FGF/FGFR signaling at the immune-stromal interface in autoimmune rheumatic diseases: from tissue remodeling to translational opportunities.Frontiers in immunology · 2026Review
- Cryoelectrospun Elastin-Alginate Scaffolds Support In Vitro 3D Epithelial-Stromal Cocultures for Salivary Tissue Engineering.Gels (Basel, Switzerland) · 2025Article
- Cryoelectrospun elastin-alginate scaffolds as potential cell delivery vehicles for mesenchymal stromal cell therapy.Scientific reports · 2025Article
- Biomimetic Catechol-Incorporated Polyacrylonitrile Nanofiber Scaffolds for Tissue Engineering of Functional Salivary Glands.Biomaterials research · 2025Article
- Salivary Gland Tissue Recombination Can Modify Cell Fate.Journal of dental research · 2024Article
- Salivary Gland Bioengineering.Bioengineering (Basel, Switzerland) · 2023Review
- Craniofacial developmental biology in the single-cell era.Development (Cambridge, England) · 2023Review
- Slow hydrogel matrix degradation enhances salivary gland mimetic phenotype.Acta biomaterialia · 2023Article
- Gene expression in organoids: an expanding horizon.Biology direct · 2023Review
- FGF signaling regulates salivary gland branching morphogenesis by modulating cell adhesion.Development (Cambridge, England) · 2023Article
- Identifying Fibrogenic Cells Following Salivary Gland Obstructive Injury.bioRxiv : the preprint server for biology · 2023Article
- Identifying fibrogenic cells following salivary gland obstructive injury.Frontiers in cell and developmental biology · 2023Article
- Hydrogels for Salivary Gland Tissue Engineering.Gels (Basel, Switzerland) · 2022Review
- Raman microspectroscopy fingerprinting of organoid differentiation state.Cellular & molecular biology letters · 2022Article
- Bioengineering in salivary gland regeneration.Journal of biomedical science · 2022Review
- Cell type-specific transforming growth factor-β (TGF-β) signaling in the regulation of salivary gland fibrosis and regeneration.Journal of oral biology and craniofacial researchArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Stromal cells can direct the differentiation of epithelial progenitor cells during organ development. Fibroblast growth factor (FGF) signaling is essential for submandibular salivary gland development. Through stromal fibroblast cells, FGF2 can indirectly regulate proacinar cell differentiation in organoids, but the mechanisms are not understood. We performed single-cell RNA-sequencing and identified multiple stromal cell subsets, including Pdgfra+ stromal subsets expressing both Fgf2 and Fgf10. When combined with epithelial progenitor cells in organoids, magnetic-activated cell-sorted PDGFRα+ cells promoted proacinar cell differentiation similarly to total stroma. Gene expression analysis revealed that FGF2 increased the expression of multiple stromal genes, including Bmp2 and Bmp7. Both BMP2 and BMP7 synergized with FGF2, stimulating proacinar cell differentiation but not branching. However, stromal cells grown without FGF2 did not support proacinar organoid differentiation and instead differentiated into myofibroblasts. In organoids, TGFβ1 treatment stimulated myofibroblast differentiation and inhibited the proacinar cell differentiation of epithelial progenitor cells. Conversely, FGF2 reversed the effects of TGFβ1. We also demonstrated that adult salivary stromal cells were FGF2 responsive and could promote proacinar cell differentiation. These FGF2 signaling pathways may have applications in future regenerative therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.