Evidence map›Paper›PMID 35222925›Full record

ArticleChemical science2022

Generation of bright monomeric red fluorescent proteins

Sandrine Legault, Derek P Fraser-Halberg, Ralph L McAnelly, Matthew G Eason, Michael C Thompson, Roberto A Chica

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sandrine LegaultDepartment of Chemistry and Biomolecular Sciences, University of Ottawa 10 Marie-Curie Ottawa Ontario K1N 6N5 Canada rchica@uottawa.ca.
Derek P Fraser-HalbergDepartment of Chemistry and Biomolecular Sciences, University of Ottawa 10 Marie-Curie Ottawa Ontario K1N 6N5 Canada rchica@uottawa.ca.ORCID https://orcid.org/0000-0002-8075-8254
Ralph L McAnellyDepartment of Chemistry and Biochemistry, University of California, Merced Merced California 95343 USA.
Matthew G EasonDepartment of Chemistry and Biomolecular Sciences, University of Ottawa 10 Marie-Curie Ottawa Ontario K1N 6N5 Canada rchica@uottawa.ca.
Michael C ThompsonDepartment of Chemistry and Biochemistry, University of California, Merced Merced California 95343 USA.
Roberto A ChicaDepartment of Chemistry and Biomolecular Sciences, University of Ottawa 10 Marie-Curie Ottawa Ontario K1N 6N5 Canada rchica@uottawa.ca.ORCID https://orcid.org/0000-0003-3789-9841

Funding

User Training and OutreachP30GM124169 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI PAUL David ADAMS, Gregory L Hura · 2017 to 2026
$28.6M
Eliminating Critical Systematic Errors In Structural Biology With Next-Generation SimulationR01GM124149 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HOLTON, JAMES M · 2017 to 2024
$2.5M
NIGMS NIH HHS P30 GM124169NIGMS NIH HHS R01 GM124149
6 · The paper itself

Abstract

Red fluorescent proteins (RFPs) have found widespread application in chemical and biological research due to their longer emission wavelengths. Here, we use computational protein design to increase the quantum yield and thereby brightness of a dim monomeric RFP (mRojoA, quantum yield = 0.02) by optimizing chromophore packing with aliphatic residues, which we hypothesized would reduce torsional motions causing non-radiative decay. Experimental characterization of the top 10 designed sequences yielded mSandy1 (

Identifiers

PMID35222925
PMCPMC8809391

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.