Evidence map›Paper›PMID 35222862›Full record

ArticleIranian journal of microbiology2021

Cell death analysis of recombinant mature epsilon toxin on the kidney cell line.

Roza Chehreara, Shohreh Zare Karizi, Hamideh Mahmoodzadeh Hosseini, Seyed Ali Mirhosseini, Mohammad Shafiei, Jafar Amani, Rouhollah Kazemi

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Iranian journal of microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Cytotoxic effects of epsilon toxin fromToxicology research · 2025
    Article
  3. Epsilon Toxin fromToxins · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Roza ChehrearaDepartment of Genetics, Faculty of Basic Sciences, Islamic Azad University, Research Branch, Tehran, Iran.
Shohreh Zare KariziDepartment of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva, Branch Islamic Azad University, Varamin, Iran.
Hamideh Mahmoodzadeh HosseiniApplied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Seyed Ali MirhosseiniApplied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Mohammad ShafieiApplied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Jafar AmaniApplied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Rouhollah KazemiDepartment of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Baqiyatallah University of Medical Sciences · IRIslamic Azad University of Varamin · IRNational Institute of Genetic Engineering and Biotechnology · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesEpsilon toxin is the third hazardous bacterial toxin causing ABS enterotoxaemia in domestic animal. In addition, epsilon toxin is known as a biological warfare agent. The aim of this study was to produce the recombinant mature epsilon toxin to evaluate cell death impact on the kidney cell line. MATERIALS AND

methodsFor this purpose, the sequence of mature epsilon toxin (46-328 aa) in pET28a was cloned and expressed in

resultsInduction by 1 mM IPTG for 4 h at 37°C was an optimized condition for expressing mature epsilon toxin in

conclusionThe recombinant mature epsilon toxin had cytotoxic effects and could induce necrosis.

Indexed as

Cell deathClostridium perfringensEpsilon toxinNecrosisRecombinant expression

Identifiers

PMID35222862
PMCPMC8816699
OpenAlexW4200244683

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.