ArticleJournal of neuroscience methods2022
A conditional GABAergic synaptic vesicle marker for Drosophila.
Article in Journal of neuroscience methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- An octopamine-glutamate cotransmitting neuron gates aggressive escalation through layered inhibition inScience advances · 2026Article
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- dFlpTag, a RMCE-based tool for simultaneous endogenous protein tagging and cell labeling in Drosophila.Scientific reports · 2025Article
- Two classes of amine/glutamate multi-transmitter neurons innervate Drosophila internal male reproductive organs.bioRxiv : the preprint server for biology · 2025Article
- Comparative Analysis of Two Autophagy-Enhancing Small Molecules (AUTEN-67 and -99) in aInternational journal of molecular sciences · 2025Article
- Rescue of Epilepsy-Associated Mutations of the Highly Conserved Glycine Residue 443 in the Human GABA Transporter 1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Pathogenic variants at the N-terminal arginine residue 44 disrupt human GABA transporter 1 function: insights fromFrontiers in pharmacology · 2025Article
- Constitutive and Conditional Epitope Tagging of Endogenous G-Protein-Coupled Receptors inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Horizontal-cell like Dm9 neurons inFrontiers in molecular neuroscience · 2024Article
- SynLight: a bicistronic strategy for simultaneous active zone and cell labeling in the Drosophila nervous system.G3 (Bethesda, Md.) · 2023Article
- SynLight: a dicistronic strategy for simultaneous active zone and cell labeling in thebioRxiv : the preprint server for biology · 2023Article
- A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses inCell reports methods · 2023Article
- Genetic regulation of central synapse formation and organization in Drosophila melanogaster.Genetics · 2022Review
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThroughout the animal kingdom, GABA is the principal inhibitory neurotransmitter of the nervous system. It is essential for maintaining the homeostatic balance between excitation and inhibition required for the brain to operate normally. Identification of GABAergic neurons and their GABA release sites are thus essential for understanding how the brain regulates the excitability of neurons and the activity of neural circuits responsible for numerous aspects of brain function including information processing, locomotion, learning, memory, and synaptic plasticity, among others. NEW
methodSince the structure and features of GABA synapses are critical to understanding their function within specific neural circuits of interest, here we developed and characterized a conditional marker of GABAergic synaptic vesicles for Drosophila, 9XV5-vGAT.
results9XV5-vGAT is validated for conditionality of expression, specificity for localization to synaptic vesicles, specificity for expression in GABAergic neurons, and functionality. Its utility for GABAergic neurotransmitter phenotyping and identification of GABA release sites was verified for ellipsoid body neurons of the central complex. In combination with previously reported conditional SV markers for acetylcholine and glutamate, 9XV5-vGAT was used to demonstrate fast neurotransmitter phenotyping of subesophageal ganglion neurons. COMPARISON WITH EXISTING
methodsThis method is an alternative to single cell transcriptomics for neurotransmitter phenotyping and can be applied to any neurons of interest represented by a binary transcription system driver.
conclusionA conditional GABAergic synaptic vesicle marker has been developed and validated for GABA neurotransmitter phenotyping and subcellular localization of GABAergic synaptic vesicles.
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