ArticleThe Plant journal : for cell and molecular biology2022
Identification of novel regulators required for early development of vein pattern in the cotyledons by single-cell RNA-sequencing.
Article in The Plant journal : for cell and molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed.
- Integration of Spatiotemporal Multi-Omics in Peach Fruit Unravels a Metabolic Niche and the Genetic Basis of Trichome-Mediated Stress Adaptation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Single-Cell Transcriptomics Reveals FLS2-Dependent Hypoxia Signaling and ERF13-Mediated Transcription During flg22-Triggered Immunity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Identification of key regulators for the development of Prunus leaves using single-cell RNA sequencing.BMC plant biology · 2026Article
- Regulatory DNA Sequences Controlling Gene Expression in Leaf Phloem Cells.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Genome-wide characterisation and expression analysis ofFrontiers in plant science · 2026Article
- Single-cell transcriptomics reveals cellular and genetic mechanisms of alpine adaptation inFrontiers in plant science · 2026Article
- Regulatory DNA Sequences Controlling Gene Expression in Leaf Phloem Cells.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Construction of a single-cell transcriptome atlas for Pogostemon cablin embryoids reveals PcNAC048 as a dual regulator coordinating lateral root morphogenesis and patchouli alcohol biosynthesis.Communications biology · 2025Article
- DOF gene family in P. sativum (L.): comprehensive genomic identification, phylogenetic examination, evolutionary growth, and expression analysis.BMC genomics · 2025Article
- Why "Where" Matters as Much as "How Much": Single-Cell and Spatial Transcriptomics in Plants.International journal of molecular sciences · 2025Review
- Transcriptomic response analysis of rubber tree saplings to water-deficit stress at single-cell resolution.Communications biology · 2025Article
- Single-cell transcriptome sequencing reveals the sphingolipid metabolism pathway plays an important role in leaf senescence in tobacco.BMC plant biology · 2025Article
- Single-cell omics in plant biology: mechanistic insights and applications for crop improvement.Advanced biotechnology · 2025Review
- Deciphering the biosynthesis pathway of gamma terpinene cuminaldehyde and para cymene in the fruit of Bunium persicum.Scientific reports · 2025Article
- Overexpression of pxAlaAT3 in Populus × xiaohei alleviates root growth inhibition under ammonium nitrogen stress.Plant cell reports · 2025Article
- Single-Cell Transcriptome Reveals the Cellular Response to PEG-Induced Stress in Wheat Leaves.Journal of agricultural and food chemistry · 2025Article
- Single-cell RNA sequencing reveals a key regulator ZmEREB14 affecting shoot apex development and yield formation in maize.Plant biotechnology journal · 2025Article
- Integrative Multi-Omics Approaches for Identifying and Characterizing Biological Elements in Crop Traits: Current Progress and Future Prospects.International journal of molecular sciences · 2025Review
- Opportunities and challenges in the application of single-cell transcriptomics in plant tissue research.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
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12 authors.
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Abstract
The leaf veins of higher plants contain a highly specialized vascular system comprised of xylem and phloem cells that transport water, organic compounds and mineral nutrients. The development of the vascular system is controlled by phytohormones that interact with complex transcriptional regulatory networks. Before the emergence of true leaves, the cotyledons of young seedlings perform photosynthesis that provides energy for the sustainable growth and survival of seedlings. However, the mechanisms underlying the early development of leaf veins in cotyledons are still not fully understood, in part due to the complex cellular composition of this tissue. To better understand the development of leaf veins, we analyzed 14 117 single cells from 3-day-old cotyledons using single-cell RNA sequencing. Based on gene expression patterns, we identified 10 clusters of cells and traced their developmental trajectories. We discovered multiple new marker genes and developmental features of leaf veins. The transcription factor networks of some cell types indicated potential roles of CYCLING DOF FACTOR 5 (CDF5) and REPRESSOR OF GA (RGA) in the early development and function of the leaf veins in cotyledons. These new findings lay a foundation for understanding the early developmental dynamics of cotyledon veins. The mechanisms underlying the early development of leaf veins in cotyledons are still not fully understood. In this study, we comprehensively characterized the early differentiation and development of leaf veins in 3-day-old cotyledons based on single-cell transcriptome analysis. We identified the cell types and novel marker genes of leaf veins and characterized the novel regulators of leaf vein.
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