Evidence map›Paper›PMID 35218587›Full record

ArticleEuropean journal of oral sciences2022

Comparability of microbiota of swabbed and spit saliva.

Amke Marije Kaan, Bernd W Brandt, Mark J Buijs, Wim Crielaard, Bart Jf Keijser, Egija Zaura

Abstract read
In one paragraph

Article in European journal of oral sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Trial
  3. Review
  4. Review
  5. Article
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  8. Review
  9. IntratumoralJournal of oral microbiology · 2025
    Article
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  13. Review
  14. Comparability of microbiota of swabbed and spit saliva.European journal of oral sciences · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Amke Marije KaanAcademic Centre for Dentistry Amsterdam, Preventive Dentistry, Amsterdam, The Netherlands.ORCID 0000-0002-9699-9271
Bernd W BrandtAcademic Centre for Dentistry Amsterdam, Preventive Dentistry, Amsterdam, The Netherlands.ORCID 0000-0002-1155-1989
Mark J BuijsAcademic Centre for Dentistry Amsterdam, Preventive Dentistry, Amsterdam, The Netherlands.ORCID 0000-0002-4972-9797
Wim CrielaardAcademic Centre for Dentistry Amsterdam, Preventive Dentistry, Amsterdam, The Netherlands.
Bart Jf KeijserAcademic Centre for Dentistry Amsterdam, Preventive Dentistry, Amsterdam, The Netherlands.
Egija ZauraAcademic Centre for Dentistry Amsterdam, Preventive Dentistry, Amsterdam, The Netherlands.ORCID 0000-0003-1432-6194

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In general, saliva is used for microbiota analysis in longitudinal studies, and several collection methods are being used. Using a robust sample collection procedure is important, as it may influence salivary composition. This study explored the comparability of the microbiota of swabbed and spit saliva. Twenty-two females participated in this cross-sectional study. The bacterial composition of the three saliva samples (swab collected by the participant (SW-P), swab collected by the researcher (SW-R), and spit (SP) was assessed by 16S rRNA gene amplicon sequencing. The bacterial composition of the swabbed and the spit saliva was significantly different irrespective of the operator, and Shannon diversity was significantly higher in spit saliva than in SW-P and SW-R. The salivary microbiota of spit and swabbed adult saliva differs significantly. Research on microbial composition therefore requires collection of similar saliva sample types in all study participants.

Indexed as

MicrobiotaSalivaAdultBacteriaCross-Sectional StudiesFemaleHumansRNA, Ribosomal, 16SRNA, Ribosomal, 16S16S rDNAmicrobiologyoral

Identifiers

PMID35218587
PMCPMC9305955

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.