Evidence map›Paper›PMID 35218540›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Quantifiable Intravital Light Sheet Microscopy.

Holly C Gibbs, Sreeja Sarasamma, Oscar R Benavides, David G Green, Nathan A Hart, Alvin T Yeh, Kristen C Maitland, Arne C Lekven

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Holly C GibbsDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, USA. hgibbs@tamu.edu.
Sreeja SarasammaDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, USA.
Oscar R BenavidesDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, USA.
David G GreenDepartment of Cell and Systems Biology, University of Toronto, Toronto, Canada.
Nathan A HartDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, USA.
Alvin T YehDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, USA.
Kristen C MaitlandDepartment of Biomedical Engineering, Texas A&M University, College Station, TX, USA.
Arne C LekvenDepartment of Biology and Biochemistry, University of Houston, Houston, TX, USA.
Texas A&M University · USUniversity of Houston · USUniversity of Toronto · CA

Funding

Imaging Dynamics and Interactions of Developmental Lineages in the Early EmbryoR01NS088564 · NINDS · TEXAS ENGINEERING EXPERIMENT STATION · PI YEH, ALVIN T · 2015 to 2018
$1.2M
Imaging enhancer-promoter interaction dynamics during embryonic brain developmentR21NS109504 · NINDS · TEXAS ENGINEERING EXPERIMENT STATION · PI GIBBS, HOLLY · 2019 to 2020
$425k
NINDS NIH HHS R01 NS088564NINDS NIH HHS R21 NS109504
6 · The paper itself

Abstract

Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently poor that computer-aided analysis or even manual analysis is not possible. Here, we describe our mounting strategy for imaging the zebrafish midbrain-hindbrain boundary (MHB) with light sheet fluorescence microscopy (LSFM) and pilot experiments to create a study-specific set of parameters for semiautomatically tracking cellular movements in the embryonic midbrain primordium during zebrafish segmentation.

Indexed as

ZebrafishZebrafish ProteinsAnimalsMesencephalonMicroscopy, FluorescenceRhombencephalonZebrafish ProteinsBioimage analysisLight sheet fluorescence microscopyMidbrain–hindbrain boundaryZebrafish

Identifiers

PMID35218540
OpenAlexW4214607780

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.