Evidence map›Paper›PMID 35218532›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Optimizing Long-Term Live Cell Imaging.

Alex Lac, Austin Le Lam, Bryan Heit

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
8.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Not So Bioorthogonal Chemistry.Journal of the American Chemical Society · 2025
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Alex LacDepartment of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada.
Austin Le LamDepartment of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada.
Bryan HeitDepartment of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada. bheit@uwo.ca.
Western University · CARobarts Clinical Trials · CA

Funding

CIHR PJT-162203
6 · The paper itself

Abstract

Live cell microscopy has become a common technique for exploring dynamic biological processes. When combined with fluorescent markers of cellular structures of interest, or fluorescent reporters of a biological activity of interest, live cell microscopy enables precise temporally and spatially resolved quantitation of the biological processes under investigation. However, because living cells are not normally exposed to light, live cell fluorescence imaging is significantly hindered by the effects of photodamage, which encompasses photobleaching of fluorophores and phototoxicity of the cells under observation. In this chapter, we outline several methods for optimizing and maintaining long-term imaging of live cells while simultaneously minimizing photodamage. This protocol demonstrates the intracellular trafficking of early and late endosomes following phagocytosis using both two and three dimensional imaging, but this protocol can easily be modified to image any biological process of interest in nearly any cell type.

Indexed as

Fluorescent DyesOptical ImagingBiological AssayMicroscopy, FluorescencePhotobleachingFluorescent DyesAcquisition parametersFluorescence microscopyIntracellular traffickingLive cell imagingPhagocytosisPhotobleachingPhotodamagePhototoxicityTime-lapsez-stack

Identifiers

PMID35218532
OpenAlexW4214550186

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.