Evidence map›Paper›PMID 35217708›Full record

ArticleScientific reports2022

Influence of extracellular protein isolated from fish gut associated bacteria as an enhancer of growth and innate immune system in Mugil cephalus.

Rajasekar Thirunavukkarasu, Priyadarshin Pandi, Deivasigamani Balaraman, Fadwa Albalawi, Naushad Ahmad, Mani Panagal, Tentu Nageswara Rao, Kumaran Subramanian, Edward Gnana Jothi George, MaryShamya Arockia Rajan and 3 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 10 citations in OpenAlex.

  1. Frontiers in veterinary science · 2025
    Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 2 countries.

Rajasekar Thirunavukkarasu *Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, 600 119, India.
Priyadarshin Pandi *Centre of Advanced Study (CAS) in Marine Biology, Annamalai University, Parangipettai, Cuddalore, Tamil Nadu, 632502, India.
Deivasigamani BalaramanCentre of Advanced Study (CAS) in Marine Biology, Annamalai University, Parangipettai, Cuddalore, Tamil Nadu, 632502, India.
Fadwa AlbalawiDepartment of Zoology, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.
Naushad AhmadChemistry Department, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.
Mani PanagalDepartment of Biotechnology, Annai College of Arts & Science, Kumbakonam, Tamil Nadu, India.
Tentu Nageswara RaoDepartment of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, 521001, India.
Kumaran SubramanianCentre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, 600 119, India.
Edward Gnana Jothi GeorgeCentre of Advanced Study (CAS) in Marine Biology, Annamalai University, Parangipettai, Cuddalore, Tamil Nadu, 632502, India.
MaryShamya Arockia RajanCentre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, 600 119, India.
Pugazhvendan Sampath RenugaArignar Anna Government Arts College, Cheyyar, Tamil Nadu, 604407, India.
Wilson AruniMusculoskeletal Research Center, The Loma Linda Veteran Affairs, United States Department of Veteran Affairs, Loma Linda, CA, 92354, US.
Suliman Yousef AlOmarChemistry Department, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia. syalomar@ksu.edu.sa.
Annamalai University · INKing Saud University · SASathyabama Institute of Science and Technology · INArignar Anna Memorial Cancer Hospital & Research Institute · INKrishna University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The cultural microbiomes of 27 bacteria colonies were isolated from Mugil cephalus for analysis of the antibacterial and antagonistic activities. A potent probiotic bacterium was characterized using16S r RNA sequencing. The potent strain was added to fish diet to perform the challenge test and to study the growth and immunological parameter. The extracellular proteins from the probiotic were collected and characterized using MALDI TOF/TOF. Out of G27, G9 strain inhibited all the five pathogenic strains. An isolated bacterium was identified as Bacillus subtilis PRBD09 with accession number KF765648. After 35 days of feeding period B. subtilis PRBD09 enhance the both cellular and humoral immune responses, which responsible for survive of the Mugil cephalus against Aeromonas hydrophila infection. The MALDI TOF sample 08 and 09 were recognized as hypothetical proteins based on the MALDI TOF sample. A cytidinedeaminase was found in samples 10, 11, and 12. Extracellular proteins may be involved for the immunological increase in Mugil cephalus against Aeromonas hydrophila, according to the current research.

Indexed as

Fish DiseasesGram-Negative Bacterial InfectionsProbioticsSmegmamorphaAeromonas hydrophilaAnimalsBacillus subtilisFishesImmune SystemImmunity, Innate

Identifiers

PMID35217708
PMCPMC8881613
OpenAlexW4214514683

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.