ArticleInternational journal of molecular sciences2022
Root Membrane Ubiquitinome under Short-Term Osmotic Stress.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Evolutionary tuning of the molecular charge state of UBP24 shapes responses to high temperature.Nature plants · 2026Article
- Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2.The Plant journal : for cell and molecular biology · 2026Article
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- The Hrd1-Mediated ERAD Pathway in Plants: Conserved Principles and Plant-Specific Innovations.International journal of molecular sciences · 2026Review
- Dynamic ASK1 proximity networks uncover SCF-dependent and noncanonical roles in ABA and drought adaptation.bioRxiv : the preprint server for biology · 2025Article
- Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis.The Plant cell · 2024Article
- A multilevel investigation to reveal the regulatory mechanism of lignin accumulation in juice sac granulation of pomelo.BMC plant biology · 2024Article
- Transcriptomics of temperature-sensitive R gene-mediated resistance identifies a WAKL10 protein interaction network.Scientific reports · 2024Article
- Characterisation of the OTU domain deubiquitinase complement ofLife science alliance · 2023Article
- Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots.Antioxidants (Basel, Switzerland) · 2022Article
- AtSnRK2.4 Functions as an ABA-Responsive Protein Kinase in Arabidopsis.Physiologia plantarumArticle
- Protein Metabolism Underlying Heat Tolerance in Contrasting Creeping Bentgrass Lines: Insights From Gel-Free Proteomics and Polyubiquitin-Omics.Physiologia plantarumArticle
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Authors and funding
10 authors at 1 institution in 2 countries.
Funding
Abstract
Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic-mediated stress. In this study, we used the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry to compile a list of 719 ubiquitinated lysine (K-Ub) residues from 450 Arabidopsis root membrane proteins (58% of which are transmembrane proteins), thereby adding to the database of ubiquitinated substrates in plants. Although no ubiquitin (Ub) motifs could be identified, the presence of acidic residues close to K-Ub was revealed. Our ubiquitinome analysis pointed to a broad role of ubiquitination in the internalization and sorting of cargo proteins. Moreover, the simultaneous proteome and ubiquitinome quantification showed that ubiquitination is mostly not involved in membrane protein degradation in response to short osmotic treatment but that it is putatively involved in protein internalization, as described for the aquaporin PIP2;1. Our in silico analysis of ubiquitinated proteins shows that two E2 Ub-conjugating enzymes, UBC32 and UBC34, putatively target membrane proteins under osmotic stress. Finally, we revealed a positive role for UBC32 and UBC34 in primary root growth under osmotic stress.
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