Evidence map›Paper›PMID 35215961›Full record

ArticleViruses2022

Selection of Primer-Template Sequences That Bind with Enhanced Affinity to Vaccinia Virus E9 DNA Polymerase.

Jeffrey J DeStefano, Frédéric Iseni, Nicolas Tarbouriech

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jeffrey J DeStefanoDepartment of Cell Biology and Molecular Genetics, Bioscience Research Building, University of Maryland, College Park, MD 20742, USA.ORCID 0000-0002-8710-7622
Frédéric IseniUnité de Virologie, Institut de Recherche Biomédicale des Armées, BP73, 91223 Brétigny-sur-Orge, France.
Nicolas TarbouriechInstitut de Biologie Structurale, Université Grenoble Alpes, Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique et aux Energies Alternatives, IBS, 38000 Grenoble, France.

Funding

Biochemistry of HIV reverse transcriptase fidelity and inhibitor interactionsR01AI150480 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI DESTEFANO, JEFFREY J · 2019 to 2019
$294k
NIAID NIH HHS R01 AI150480
6 · The paper itself

Abstract

A modified SELEX (Systematic Evolution of Ligands by Exponential Enrichment) pr,otocol (referred to as PT SELEX) was used to select primer-template (P/T) sequences that bound to the vaccinia virus polymerase catalytic subunit (E9) with enhanced affinity. A single selected P/T sequence (referred to as E9-R5-12) bound in physiological salt conditions with an apparent equilibrium dissociation constant (K

Indexed as

Avian Myeloblastosis VirusBase SequenceDNA-Directed DNA PolymeraseDNA, ViralHIV Reverse TranscriptaseMoloney murine leukemia virusProtein BindingSELEX Aptamer TechniqueVaccinia virusViral ProteinsVirus ReplicationDNA-Directed DNA PolymeraseDNA polymerase, vaccinia virusDNA, ViralHIV Reverse TranscriptaseViral ProteinsDNA polymerase sequence preferenceE9SELEXvaccinia virus polymerase

Identifiers

PMID35215961
PMCPMC8880465

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.