Evidence map›Paper›PMID 35215880›Full record

ArticleViruses2022

Visualization of Marek's Disease Virus Genomes in Living Cells during Lytic Replication and Latency.

Tereza Vychodil, Darren J Wight, Mariana Nascimento, Fabian Jolmes, Thomas Korte, Andreas Herrmann, Benedikt B Kaufer

Abstract read
In one paragraph

Article in Viruses, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tereza VychodilInstitut für Virologie, Freie Universität Berlin, Robert von Ostertag-Straße 7-13, 14163 Berlin, Germany.
Darren J WightInstitut für Virologie, Freie Universität Berlin, Robert von Ostertag-Straße 7-13, 14163 Berlin, Germany.ORCID 0000-0001-6320-5597
Mariana NascimentoInstitut für Virologie, Freie Universität Berlin, Robert von Ostertag-Straße 7-13, 14163 Berlin, Germany.ORCID 0000-0002-2208-9566
Fabian JolmesDepartment of Biology, Molecular Biophysics, Humboldt-Universität zu Berlin, Invalidenstraße 42, 10115 Berlin, Germany.ORCID 0000-0002-2005-8002
Thomas KorteDepartment of Biology, Molecular Biophysics, Humboldt-Universität zu Berlin, Invalidenstraße 42, 10115 Berlin, Germany.
Andreas HerrmannDepartment of Biology, Molecular Biophysics, Humboldt-Universität zu Berlin, Invalidenstraße 42, 10115 Berlin, Germany.ORCID 0000-0002-6716-2026
Benedikt B KauferInstitut für Virologie, Freie Universität Berlin, Robert von Ostertag-Straße 7-13, 14163 Berlin, Germany.ORCID 0000-0003-1328-2695

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Visualization of the herpesvirus genomes during lytic replication and latency is mainly achieved by fluorescence in situ hybridization (FISH). Unfortunately, this technique cannot be used for the real-time detection of viral genome in living cells. To facilitate the visualization of the Marek's disease virus (MDV) genome during all stages of the virus lifecycle, we took advantage of the well-established tetracycline operator/repressor (TetO/TetR) system. This system consists of a fluorescently labeled TetR (TetR-GFP) that specifically binds to an array of

Indexed as

Genome, ViralVirus LatencyVirus ReplicationAnimalsBacterial ProteinsCarrier ProteinsCell NucleusCells, CulturedChickensGiant CellsGreen Fluorescent ProteinsHerpesvirus 2, GallidRepressor ProteinsT-LymphocytesViral Replication CompartmentsBacterial ProteinsCarrier ProteinsGreen Fluorescent ProteinsRepressor ProteinsTet O resistance protein, Bacteriatetracycline resistance-encoding transposon repressor proteingenome integrationlatencylive-cell genome visualizationlytic replicationMarek’s disease virusT cellsTetO/TetR system

Identifiers

PMID35215880
PMCPMC8877148

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.