ArticlePathogens (Basel, Switzerland)2022
Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses.
Article in Pathogens (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- A Look Under the Carpet of a Successful Eradication Campaign Against Small Ruminant Lentiviruses.Pathogens (Basel, Switzerland) · 2025Article
- Preliminary study on paratuberculosis, small ruminant lentivirus, and co-infection in Emilia Romagna flocks.Open veterinary journal · 2025Article
- Molecular Characterization of Small Ruminant Lentiviruses in Sheep and Goats: A Systematic Review.Animals : an open access journal from MDPI · 2024Review
- First Molecular Characterization of Small Ruminant Lentiviruses in Hungarian Goat Population.Pathogens (Basel, Switzerland) · 2024Article
- Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses.Journal of veterinary research · 2024Article
- Detection of small ruminant Lentivirus proviral DNA in red deer from Poland.BMC veterinary research · 2024Article
- In vivo evaluation of the antiretroviral activity of Melia azedarach against small ruminant lentiviruses in goat colostrum and milk.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- The genetic variability of small-ruminant lentiviruses and its impact on tropism, the development of diagnostic tests and vaccines and the effectiveness of control programmes.Journal of veterinary research · 2023Article
- First Molecular Characterization of Small Ruminant Lentiviruses Detected in Romania.Animals : an open access journal from MDPI · 2023Article
- Isolation and Identification of Caprine Arthritis Encephalitis Virus from Animals in the Republic of Mordovia.Animals : an open access journal from MDPI · 2023Article
- Article
- Longitudinal Study on Seroreactivity of Goats Exposed to Colostrum and Milk of Small Ruminant Lentivirus-infected Dams.Journal of veterinary research · 2022Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small ruminant lentiviruses (SRLVs), i.e., CAEV and MVV, cause insidious infections with life-long persistence and a slowly progressive disease, impairing both animal welfare and productivity in affected herds. The complex diagnosis of SRLVs currently combines serological methods including whole-virus and peptide-based ELISAs and Immunoblot. To improve the current diagnostic protocol, we analyzed 290 sera of animals originating from different European countries in parallel with three commercial screening ELISAs, Immunoblot as a confirmatory assay and five SU5 peptide ELISAs for genotype differentiation. A newly developed nested real-time PCR was carried out for the detection and genotype differentiation of the virus. Using a heat-map display of the combined results, the drawbacks of the current techniques were graphically visualized and quantified. The immunoblot and the SU5-ELISAs exhibited either unsatisfactory sensitivity or insufficient reliability in the differentiation of the causative viral genotype, respectively. The new truth standard was the concordance of the results of two out of three screening ELISAs and the PCR results for serologically false negative samples along with genotype differentiation. Whole-virus antigen-based ELISA showed the highest sensitivity (92.2%) and specificity (98.9%) among the screening tests, whereas PCR exhibited a sensitivity of 75%.
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