Evidence map›Paper›PMID 35212991›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Culture of Mouse Liver Ductal Organoids.

Junkai Yan, Yunling Tai, Huiping Zhou

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Junkai YanDepartment of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Central Virginia Veterans Health System, Richmond, VA, USA.
Yunling TaiDepartment of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Central Virginia Veterans Health System, Richmond, VA, USA.
Huiping ZhouDepartment of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Central Virginia Veterans Health System, Richmond, VA, USA. huiping.zhou@vcuhealth.org.

Funding

Regulation of Hepatic SphK2 by Bile Acids: Effects on Lipid MetabolismR01DK057543 · NIDDK · VIRGINIA COMMONWEALTH UNIVERSITY · PI HYLEMON, PHILLIP B, ZHOU, HUIPING ROSE · 2001 to 2015
$4.4M
LncRNA H19 in Cholestatic Liver DiseasesR01DK115377 · NIDDK · VIRGINIA COMMONWEALTH UNIVERSITY · PI HYLEMON, PHILLIP B, ZHOU, HUIPING ROSE · 2018 to 2021
$2.1M
Bile Acid and Sphingosine-1-phosphate Receptor-mediated Signaling in CholestasisR01DK104893 · NIDDK · VIRGINIA COMMONWEALTH UNIVERSITY · PI HYLEMON, PHILLIP B, ZHOU, HUIPING ROSE · 2016 to 2020
$2.0M
Sphingosine-1 phosphate signaling in alcoholic liver diseaseR21AA026629 · NIAAA · VIRGINIA COMMONWEALTH UNIVERSITY · PI ZHOU, HUIPING ROSE · 2019 to 2020
$407k
The ShEEP request for NanoString GeoMx Digital Spatial Profiling and nCounter MAX Complete SystemIS1BX005517 · VA · VA VETERANS ADMINISTRATION HOSPITAL · PI ZHOU, HUIPING ROSE · 2021 to 2021
–
The ShEEP request for Waters Xevo TQ-XS Liquid Chromatography Mass Spectrometer SystemIS1BX004777 · VA · VA VETERANS ADMINISTRATION HOSPITAL · PI ZHOU, HUIPING ROSE · 2019 to 2019
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BLR&D Research Career Scientist Award ApplicationIK6BX004477 · VA · VA VETERANS ADMINISTRATION HOSPITAL · PI HUIPING ZHOU · 2019 to 2026
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Mechanisms of Berberine for the Treatment of Non-Alcoholic Fatty Liver DiseaseI01BX004033 · VA · VA VETERANS ADMINISTRATION HOSPITAL · PI ZHOU, HUIPING ROSE · 2018 to 2021
–
BLRD VA I01 BX004033BLRD VA IK6 BX004477BLRD VA IS1 BX004777BLRD VA IS1 BX005517NIAAA NIH HHS R21 AA026629NIDDK NIH HHS R01 DK057543NIDDK NIH HHS R01 DK104893NIDDK NIH HHS R01 DK115377
6 · The paper itself

Abstract

3D organoid culture has become a powerful tool and model for various human diseases, including liver diseases, such as non-alcoholic steatohepatitis (NASH). Hepatic organoids have significant advantages over traditional primary cell cultures. The hepatic progenitor cells can be induced to form hepatic organoids. The established organoids can be passaged or cryopreserved for future use. The established hepatic organoids can be manipulated to study the disease progression of NASH-related fibrosis. Here, we describe a protocol to establish mouse liver ductal organoids.

Indexed as

Non-alcoholic Fatty Liver DiseaseOrganoidsAnimalsLiverMiceStem CellsBile ductCholangiocytesHepatocytesLiverOrganoids

Identifiers

PMID35212991
PMCPMC9327439

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.