Evidence map›Paper›PMID 35211956›Full record

ArticleBiotechnology and bioengineering2022

Inexpensive protein overexpression driven by the NarL transcription activator protein.

Joanne Hothersall, Sandie Lai, Nan Zhang, Rita E Godfrey, Patcharawarin Ruanto, Sarah Bischoff, Colin Robinson, Tim W Overton, Stephen J W Busby, Douglas F Browning

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology and bioengineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Optimisation of recombinant TNFα production inFrontiers in bioengineering and biotechnology · 2023
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Joanne HothersallSchool of Biosciences, Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.
Sandie LaiSchool of Chemical Engineering, University of Birmingham, Birmingham, UK.
Nan ZhangSchool of Biosciences, Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.
Rita E GodfreySchool of Biosciences, Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.
Patcharawarin RuantoSchool of Biosciences, Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.
Sarah BischoffSchool of Biosciences, University of Kent, Canterbury, UK.
Colin RobinsonSchool of Biosciences, University of Kent, Canterbury, UK.ORCID 0000-0002-1739-032X
Tim W OvertonSchool of Chemical Engineering, University of Birmingham, Birmingham, UK.ORCID 0000-0003-3050-2549
Stephen J W BusbySchool of Biosciences, Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.
Douglas F BrowningSchool of Biosciences, Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.ORCID 0000-0003-4672-3514
University of Birmingham · GBUniversity of Kent · GBAston University · GBUniversity of Warwick · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Most Escherichia coli overexpression vectors used for recombinant protein production (RPP) depend on organic inducers, for example, sugars or simple conjugates. However, these can be expensive and, sometimes, chemically unstable. To simplify this and to cut the cost of RPP, we have developed vectors controlled by the Escherichia coli nitrate-responsive NarL transcription activator protein, which use nitrate, a cheap, stable, and abundant inorganic ion, to induce high-level controlled RPP. We show that target proteins, such as green fluorescent protein, human growth hormone, and single-chain variable region antibody fragments can be expressed to high levels using our promoter systems. As nitrate levels are high in many commercial fertilizers, we demonstrate that controlled RPP can be achieved using readily available and inexpensive garden products.

Indexed as

Escherichia coli ProteinsBase SequenceDNA-Binding ProteinsEscherichia coliGene Expression Regulation, BacterialHumansNitratesOperonTranscription FactorsTranscription, GeneticDNA-Binding ProteinsEscherichia coli ProteinsNarL protein, E coliNitratesTranscription FactorsbiopharmaceuticalsEscherichia colirecombinant protein productiontranscription regulation

Identifiers

PMID35211956
PMCPMC9314961
OpenAlexW4214493094

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.