Evidence map›Paper›PMID 35211812›Full record

ArticleBiogerontology2022

Multispectral autofluorescence characteristics of reproductive aging in old and young mouse oocytes.

Jared M Campbell, Saabah B Mahbub, Michael J Bertoldo, Abbas Habibalahi, Dale M Goss, William L Ledger, Robert B Gilchrist, Lindsay E Wu, Ewa M Goldys

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biogerontology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

  1. Pooled it
  2. Review
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  7. Article
  8. Article
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  11. Review
  12. Ovarian aging: energy metabolism of oocytes.Journal of ovarian research · 2024
    Review
  13. Article
  14. Article
  15. Label-Free Optical Metabolic Imaging in Cells and Tissues.Annual review of biomedical engineering · 2023
    Review
  16. Article
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Jared M CampbellARC Centre of Excellence Centre for Nanoscale Biophotonics, Graduate School of Biomedical Engineering, University of New South Wales Sydney, Kensington, Sydney, NSW, 2052, Australia. j.campbell@unsw.edu.au.ORCID 0000-0003-0163-4251
Saabah B MahbubARC Centre of Excellence Centre for Nanoscale Biophotonics, Graduate School of Biomedical Engineering, University of New South Wales Sydney, Kensington, Sydney, NSW, 2052, Australia.
Michael J BertoldoDiscipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, Sydney, Australia.
Abbas HabibalahiARC Centre of Excellence Centre for Nanoscale Biophotonics, Graduate School of Biomedical Engineering, University of New South Wales Sydney, Kensington, Sydney, NSW, 2052, Australia.
Dale M GossSchool of Medical Sciences, University of New South Wales Sydney, Sydney, Australia.
William L LedgerDiscipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, Sydney, Australia.
Robert B GilchristDiscipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, Sydney, Australia.
Lindsay E WuSchool of Medical Sciences, University of New South Wales Sydney, Sydney, Australia.
Ewa M GoldysARC Centre of Excellence Centre for Nanoscale Biophotonics, Graduate School of Biomedical Engineering, University of New South Wales Sydney, Kensington, Sydney, NSW, 2052, Australia.
UNSW Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Increasing age has a major detrimental impact on female fertility, which, with an ageing population, has major sociological implications. This impact is primarily mediated through deteriorating quality of the oocyte. Deteriorating oocyte quality with biological age is the greatest rate-limiting factor to female fertility. Here we have used label-free, non-invasive multi-spectral imaging to identify unique autofluorescence profiles of oocytes from young and aged animals. Discriminant analysis demonstrated that young oocytes have a distinct autofluorescent profile which accurately distinguishes them from aged oocytes. We recently showed that treatment with the nicotinamide adenine dinucleotide (NAD+) precursor nicotinamide mononucleotide (NMN) restored oocyte quality and fertility in aged animals, and when our analysis was applied to oocytes from aged animals treated with NMN, 85% of these oocytes were classified as having the autofluorescent signature of young animals. Spectral unmixing using the Robust Dependent Component Analysis (RoDECA) algorithm demonstrated that NMN treatment altered the metabolic profile of oocytes, increasing free NAD(P)H, protein bound NAD(P)H, redox ratio and the ratio of bound to free NAD(P)H. The frequency of oocytes with simultaneously high NAD(P)H and flavin content was also significantly increased in mice treated with NMN. Young and Aged + NMN oocytes had a smoother spectral distribution, with the distribution of NAD(P)H in young oocytes specifically differing from that of aged oocytes. Identifying the multispectral profile of oocyte autofluorescence during aging could have utility as a non-invasive and sensitive measure of oocyte quality.

Indexed as

NADOocytesAgingAnimalsFemaleFertilityMiceNicotinamide MononucleotideNADNicotinamide MononucleotideAgeingAutofluorescenceHyperspectralMultispectralOocyteReproductive healthcare

Identifiers

PMID35211812
PMCPMC9023381
OpenAlexW4213424516

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.