ArticleMicroorganisms2022
Culture-Free Enumeration of
Article in Microorganisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07018076 (Candidate Clinical Correlate of Prognostic Outcome for TB Study), which is not on this map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Candidate Clinical Correlate of Prognostic Outcome for TB Study
Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Immortal genome assumption significantly underestimates replication and death rates ofMicrobiology spectrum · 2026Article
- Immortal genome assumption significantly underestimates replication and death rates ofbioRxiv : the preprint server for biology · 2026Article
- CountFU: Ditching CFUs for qPCR enumeration of Mycobacterium tuberculosis and a call to join the revolution.Tuberculosis (Edinburgh, Scotland) · 2025Article
- Deep Amplicon Sequencing Reveals Culture-dependent Clonal Selection of Mycobacterium tuberculosis in Clinical Samples.Genomics, proteomics & bioinformatics · 2025Article
- Clinically encountered growth phenotypes of tuberculosis-causing bacilli and theirFrontiers in cellular and infection microbiology · 2022Review
- Differentially culturable tubercle bacteria as a measure of tuberculosis treatment response.Frontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundThe turnaround times for phenotypic tests used to monitor the bacterial load of
objectivesWe report the adaptation of our robust, culture-free assay utilising 16S ribosomal RNA, developed for sputum, to enumerate the number of bacteria present in animal tissues as a tool to improve the read-outs in preclinical drug efficacy studies.
methodsInitial assay adaptation was performed using naïve mouse lungs spiked with known quantities of
resultsWhen tested on lung tissues derived from infected mice, the MBL assay produced comparable results to the bacterial counts in solid culture (colony forming units: CFU). Notably, under specific drug treatments, the MBL assay was able to detect a significantly higher number of
conclusionsThe MBL assay can enumerate the bacterial load in animal tissues in real time without the need to wait for extended periods for cultures to grow. The readout correlates well with CFUs. Importantly, we have shown that the MBL is able to measure specific populations of bacteria not cultured on solid agar. The adaptation of this assay for preclinical studies has the potential to decrease the readout time of data acquisition from animal experiments and could represent a valuable tool for tuberculosis drug discovery and development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.