Evidence map›Paper›PMID 35208411›Full record

ArticleMicromachines2022

Acetylcholinesterase Activity Influenced by Lipid Membrane Area and Surface Acoustic Waves.

Lukas G Schnitzler, Kathrin Baumgartner, Anna Kolb, Benedikt Braun, Christoph Westerhausen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micromachines, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lukas G SchnitzlerExperimental Physics I, Institute of Physics, University of Augsburg, 86159 Augsburg, Germany.
Kathrin BaumgartnerExperimental Physics I, Institute of Physics, University of Augsburg, 86159 Augsburg, Germany.
Anna KolbExperimental Physics I, Institute of Physics, University of Augsburg, 86159 Augsburg, Germany.
Benedikt BraunExperimental Physics I, Institute of Physics, University of Augsburg, 86159 Augsburg, Germany.ORCID 0000-0001-5504-624X
Christoph WesterhausenCenter for NanoScience (CeNS), Ludwig-Maximilians-Universität Munich, 80799 Munich, Germany.ORCID 0000-0001-7103-7060

Funding

Center for NanoScience N/AUniversity of Augsburg N/A
6 · The paper itself

Abstract

According to the current model of nerve propagation, the function of acetylcholinesterase (AChE) is to terminate synaptic transmission of nerve signals by hydrolyzing the neurotransmitter acetylcholine (ACh) in the synaptic cleft to acetic acid (acetate) and choline. However, extra-synaptic roles, which are known as 'non-classical' roles, have not been fully elucidated. Here, we measured AChE activity with the enzyme bound to lipid membranes of varying area per enzyme in vitro using the Ellman assay. We found that the activity was not affected by density fluctuations in a supported lipid bilayer (SLB) induced by standing surface acoustic waves. Nevertheless, we found twice as high activity in the presence of small unilamellar vesicles (SUV) compared to lipid-free samples. We also showed that the increase in activity scaled with the available membrane area per enzyme.

Indexed as

acetylcholinesteraseEllman assayenzyme activitylipid membranesurface acoustic waves

Identifiers

PMID35208411
PMCPMC8877910

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.