Effect of Microcystin-LR, Nodularin, Anatoxin-a, β-N-Methylamino-L-Alanine and Domoic Acid on Antioxidant Properties of Glutathione.

Cyanobacteria produce a range of toxic secondary metabolites that affect many processes in human, animal and also plant cells. In recent years, some efforts have concentrated on deepening the understanding of their effect on living cells in the context of the disruption of antioxidant systems. Many results suggest that cyanotoxins interfere with glutathione (GSH) metabolism, which often leads to oxidative stress and, in many cases, cell death. Knowledge about the influence of cyanotoxins on enzymes involved in GSH synthesis or during its antioxidant action is relatively broad. However, to date, there is no information about the antioxidant properties of GSH after its direct interaction with cyanotoxins. In this paper, we investigated the effect of four cyanotoxins belonging to the groups of hepatotoxins (microcystin-LR and nodularin) or neurotoxins (anatoxin-a and β-N-methylamino-L-alanine) on the in vitro antioxidant properties of GSH. Moreover, the same study was performed for domoic acid (DA) produced by some diatoms. The obtained results showed that none of the studied compounds had an effect on GSH antioxidant potential. The results presented in this paper are, to the best of our knowledge, the first description of the kinetics of scavenging radicals by GSH reactions under the influence of these cyanotoxins and DA. This work provides new and valuable data that broadens the knowledge of the impact of cyanotoxins and DA on GSH metabolism and complements currently available information. Future studies should focus on the effects of the studied compounds on antioxidant systems in vivo.