ArticleProceedings of the National Academy of Sciences of the United States of America1986
Developmental origins of skeletal muscle fibers: clonal analysis of myogenic cell lineages based on expression of fast and slow myosin heavy chains.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed.
- Ets2 Is an Activity-Dependent Transcriptional Regulator of a Fast-Twitch Muscle Program.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Effects of variable incubation temperature during embryonic days 10-13 on leg muscle transcriptomics in day-old broiler and layer chicks.Scientific reports · 2026Article
- Low-dose MyoAAV 2A-mediated delivery of engineered micro-utrophin achieves pan- muscle tissue distribution with elevated muscle function in Duchenne muscular dystrophy.Journal of translational medicine · 2026Article
- Single-cell transcriptomics reveal mechanisms of skeletal muscle differentiation across duck embryonic development.Communications biology · 2026Article
- Early lineage segregation of primary myotubes from secondary myotubes and adult muscle stem cells.Nature communications · 2025Article
- Review
- Myostatin gene role in regulating traits of poultry species for potential industrial applications.Journal of animal science and biotechnology · 2024Review
- Article
- Developmental, physiologic and phylogenetic perspectives on the expression and regulation of myosin heavy chains in mammalian skeletal muscles.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Review
- The Effect of Mechanical Stretch on Myotube Growth Suppression by Colon-26 Tumor-Derived Factors.Frontiers in cell and developmental biology · 2021Article
- Effects of PCB126 on Adipose-to-Muscle Communication in anEnvironmental health perspectives · 2020Article
- The effects of lead on the development of somites in chick embryos (Toxicology research · 2019Article
- EMX2 activates slow myosin heavy chain 2 gene expression in embryonic muscle fibers.Mechanisms of development · 2017Article
- Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.Nature protocols · 2016Article
- Muscle fiber type specific activation of the slow myosin heavy chain 2 promoter by a non-canonical E-box.Biochemical and biophysical research communications · 2016Article
- Genome-wide expression analysis and EMX2 gene expression in embryonic myoblasts committed to diverse skeletal muscle fiber type fates.Developmental dynamics : an official publication of the American Association of Anatomists · 2013Article
- Excitation-transcription coupling in skeletal muscle: the molecular pathways of exercise.Biological reviews of the Cambridge Philosophical Society · 2011Review
- Lineage-based primary muscle fiber type diversification independent of MEF2 and NFAT in chick embryos.Journal of muscle research and cell motility · 2011Article
- Muscle fiber type specific induction of slow myosin heavy chain 2 gene expression by electrical stimulation.Experimental cell research · 2010Article
- Prdm1 (Blimp-1) and the expression of fast and slow myosin heavy chain isoforms during avian myogenesis in vitro.PloS one · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
A clonal analysis was used to show that skeletal muscle myoblasts are committed to distinct cell lineages during development. Myoblasts taken from embryonic chicken hindlimb muscles of different ages were cultured at clonal density. The content of fast and slow classes of the myosin heavy chain isoforms in the myotubes of the resulting muscle colonies was determined immunocytochemically with specific monoclonal antibodies that served as markers for the different fiber types. The muscle colonies formed by cloning myoblasts from early hindlimbs (days 4-6 in ovo) were of three types: the most numerous type, in which all myotubes in a colony contained only the fast class of myosin heavy chain; a less numerous type, in which all myotubes in a colony contained both the fast and slow classes of myosin heavy chain isoforms; and a rare type, in which all myotubes in a colony contained only the slow class of myosin heavy chain. The muscle colonies formed by cloning myoblasts from later hindlimbs (days 10-12 in ovo) were, however, all of one type, in which every myotube in a colony contained only fast myosin heavy chain. Thus, myoblasts in the early embryo (days 4-6 in ovo) were a heterogeneous population committed to three myogenic lineages: fast, mixed fast/slow, and slow, whereas myoblasts from the later embryo (days 10-12 in ovo) were only in the fast myogenic lineage. These results suggest that muscle fiber formation is rooted in two developmental phases--an early phase in which diverse fiber types are formed from intrinsically diverse populations of myoblasts and a later phase in which fibers are formed from a single population of myoblasts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.