Evidence map›Paper›PMID 35205382›Full record

ArticleGenes2022

FDDM1 and FDDM2, Two SGS3-like Proteins, Function as a Complex to Affect DNA Methylation in Arabidopsis.

Shengjun Li, Weilong Yang, Yunfeng Liu, Guangyong Li, Xiang Liu, Yaling Liu, James R Alfano, Chi Zhang, Bin Yu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Shengjun LiKey Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Shandong Energy Institute, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.ORCID 0000-0003-0469-3222
Weilong YangCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Yunfeng LiuCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.ORCID 0000-0002-4166-9056
Guangyong LiCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Xiang LiuCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Yaling LiuCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
James R AlfanoCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Chi ZhangCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Bin YuCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
University of Nebraska–Lincoln · US

Funding

Understand the function of the MOS4-associated complex in microRNA biogenesisR01GM127414 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI YU, BIN · 2018 to 2022
$1.6M
NIGMS NIH HHS R01 GM127414
6 · The paper itself

Abstract

DNA methylation is an important epigenetic modification required for the specific regulation of gene expression and the maintenance of genome stability in plants and animals. However, the mechanism of DNA demethylation remains largely unknown. Here, we show that two SGS3-like proteins, FACTOR OF DNA DEMETHYLATION 1 (FDDM1) and FDDM2, negatively affect the DNA methylation levels at ROS1-dependend DNA loci in Arabidopsis. FDDM1 binds dsRNAs with 5' overhangs through its XS (rice gene X and SGS3) domain and forms a heterodimer with FDDM2 through its XH (rice gene X Homology) domain. A lack of FDDM1 or FDDM2 increased DNA methylation levels at several ROS1-dependent DNA loci. However, FDDM1 and FDDM2 may not have an additive effect on DNA methylation levels. Moreover, the XS and XH domains are required for the function of FDDM1. Taken together, these results suggest that FDDM1 and FDDM2 act as a heterodimer to positively modulate DNA demethylation. Our finding extends the function of plant-specific SGS3-like proteins.

Indexed as

ArabidopsisArabidopsis ProteinsDNADNA DemethylationDNA MethylationGene Expression Regulation, PlantMutationProtein-Tyrosine KinasesProto-Oncogene ProteinsArabidopsis ProteinsDNAProtein-Tyrosine KinasesProto-Oncogene ProteinsSGS3 protein, ArabidopsisArabidopsisDNA demethylationepigeneticsFDDM1 and FDDM2SGS3-like protein

Identifiers

PMID35205382
PMCPMC8872474
OpenAlexW4213302873

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.