ArticleAntioxidants (Basel, Switzerland)2022
Probing Cell Redox State and Glutathione-Modulating Factors Using a Monochlorobimane-Based Microplate Assay.
Article in Antioxidants (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
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- Anti-Photoaging Effect of Soluble Microneedles Loaded with Hydroxytyrosol.International journal of molecular sciences · 2026Article
- Cytochrome c Oxidase Subunit COX4-1 Reprograms Erastin-Induced Cell Death from Ferroptosis to Apoptosis: A Transmitochondrial Study.Antioxidants (Basel, Switzerland) · 2025Article
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- Protective Effect of Field Horsetail Polyphenolic Extract on Erythrocytes and Their Membranes.International journal of molecular sciences · 2025Article
- Exploring the role of oxidative stress and mitochondrial dysfunction in β-damascone-induced aneuploidy.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2024Article
- Carbon Monoxide: A Pleiotropic Redox Regulator of Life and Death.Antioxidants (Basel, Switzerland) · 2024Review
- Design and Synthesis of Novel Antioxidant 2-Substituted-5,7,8-Trimethyl-1,4-Benzoxazine Hybrids: Effects on Young and Senescent Fibroblasts.Antioxidants (Basel, Switzerland) · 2024Article
- Biological Activities ofInternational journal of molecular sciences · 2023Article
- Characterization of Glutathione Dithiophosphates as Long-Acting HInternational journal of molecular sciences · 2023Article
- Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior.Micromachines · 2023Article
- Compounds Inhibiting Noppera-bo, a GlutathioneBiomolecules · 2023Review
- A new triphenylphosphonium-conjugated amphipathic cationic peptide with improved cell-penetrating and ROS-targeting properties.Current research in pharmacology and drug discovery · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Thiol compounds including predominantly glutathione (GSH) are key components of redox homeostasis, which are involved in the protection and regulation of mammalian cells. The assessment of cell redox status by means of in situ analysis of GSH in living cells is often preferable over established assays in cell lysates due to fluctuations of the GSH pool. For this purpose, we propose a microplate assay with monochlorobimane (MCB) as an available fluorescent probe for GSH, although poorly detected in the microplate format. In addition to the new procedure for improved MCB-assisted GSH detection in plate-grown cells and its verification with GSH modulators, this study provides a useful methodology for the evaluation of cell redox status probed through relative GSH content and responsiveness to both supplemented thiols and variation in oxygen pressure. The roles of extracellular interactions of thiols and natural variability of cellular glutathione on the assay performance were emphasized and discussed. The results are of broad interest in cell biology research and should be particularly useful for the characterization of pathological cells with decreased GSH status and increased oxidative status as well as redox-modulating factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.