ArticleBiomedicines2022
Decellularized Dental Pulp, Extracellular Vesicles, and 5-Azacytidine: A New Tool for Endodontic Regeneration.
Article in Biomedicines, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Efficacy of dental stem cell-derived exosomes for pulp regeneration: a systematic review of clinical, animal, and in vitro studies.Molecular biology reports · 2026Pooled it
- Dose-dependent effects of Metformin on proliferation and odontogenic differentiation of dental pulp stem cells.BMC oral health · 2026Article
- Bioactive photo-crosslinkable hydrogel based on decellularized dental pulp matrix for functional pulp regeneration.BMC oral health · 2026Article
- Safety Assessment of Extracellular Vesicle-Based Therapy in Regenerative Dentistry.International journal of molecular sciences · 2026Review
- Decellularized dental pulp extracellular matrix for pulp-dentin regeneration: a narrative review of processing, bioactivity, delivery, preclinical evidence, and translational challenges.Frontiers in dental medicine · 2026Review
- Three-Dimensional Models of the Dental Pulp: Bridging Fundamental Biology and Regenerative Therapy.International journal of molecular sciences · 2025Review
- Biological and Metabolomic Characterization of Human Dermal Fibroblasts and Mesenchymal Stem Cells Derived from Human Dental Pulp and Adipose Tissue: a Pilot Comparative Study.Stem cell reviews and reports · 2025Article
- ECM-Preserving Decellularization of Dermis via Subtle Cell Membrane Disruption Induced by Subcritical Dimethyl Ether.ACS omega · 2025Article
- Mechanistic insights into dental stem cells-derived exosomes in regenerative endodontics.International endodontic journal · 2025Review
- Stem Cells in Regenerative Medicine: A Journey from Adult Stem Cells to Induced Pluripotent Cells.International journal of molecular sciences · 2025Review
- Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025Review
- Polystyrene substrate effects on human dental pulp cell behavior: implications for in vitro culture optimization.BMC oral health · 2025Article
- Effects of simulated microgravity on dental pulp stem cell stemness.Journal of molecular histology · 2025Article
- Expanding the Applicability of Electroactive Polymers for Tissue Engineering Through Surface Biofunctionalization.Biomimetics (Basel, Switzerland) · 2025Article
- DMP1-mediated transformation of DPSCs to CD31Frontiers in cell and developmental biology · 2025Article
- Extracellular vesicles as therapeutic tools in regenerative dentistry.Stem cell research & therapy · 2024Review
- Stem Cells in Bone Tissue Engineering: Progress, Promises and Challenges.Stem cell reviews and reports · 2024Review
- ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells.Heliyon · 2024Article
- Autologous hGMSC-Derived iPS: A New Proposal for Tissue Regeneration.International journal of molecular sciences · 2024Article
- Geometrically modified bovine pericardium membrane promotes the expression of molecules targeted for a faster integration and vascularization process.Frontiers in bioengineering and biotechnology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dental pulp is a major component of the dental body that serves to maintain the tooth life and function. The aim of the present work was to develop a system that functions as a growth-permissive microenvironment for dental pulp regeneration using a decellularized dental pulp (DDP) matrix, 5-Aza-2'-deoxycytidine (5-Aza), and Extracellular Vesicles (EVs) derived from human Dental Pulp Stem Cells (hDPSCs). Human dental pulps extracted from healthy teeth, scheduled to be removed for orthodontic purpose, were decellularized and then recellularized with hDPSCs. The hDPSCs were seeded on DDP and maintained under different culture conditions: basal medium (CTRL), EVs, 5-Aza, and EVs+-5-Aza. Immunofluorescence staining and Western blot analyses were performed to evaluate the proteins' expression related to dentinogenesis, such as ALP, RUNX2, COL1A1, Vinculin, DMP1, and DSPP. Protein contents found in the DDP recellularized with hDPSCs were highly expressed in samples co-treated with EVs and 5-Aza compared to other culture conditions. This study developed a DDP matrix loaded by hDPSCs in co-treatment with EVs, which might enhance the dentinogenic differentiation with a high potentiality for endodontic regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.