Evidence map›Paper›PMID 35202345›Full record

ArticleVeterinary sciences2022

Multiplexed Genome Editing for Efficient Phenotypic Screening in Zebrafish.

Shuyu Guo, Ge Gao, Cuizhen Zhang, Gang Peng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Shuyu GuoState Key Laboratory of Medical Neurobiology, Ministry of Education Frontiers Center for Brain Science, and Institution of Brain Science, Fudan University, Shanghai 200032, China.
Ge GaoState Key Laboratory of Medical Neurobiology, Ministry of Education Frontiers Center for Brain Science, and Institution of Brain Science, Fudan University, Shanghai 200032, China.
Cuizhen ZhangState Key Laboratory of Medical Neurobiology, Ministry of Education Frontiers Center for Brain Science, and Institution of Brain Science, Fudan University, Shanghai 200032, China.
Gang PengState Key Laboratory of Medical Neurobiology, Ministry of Education Frontiers Center for Brain Science, and Institution of Brain Science, Fudan University, Shanghai 200032, China.
Fudan University · CN

Funding

National Key Research and Development Program of China 2018YFA0801000
6 · The paper itself

Abstract

Zebrafish are widely used to investigate candidate genes for human diseases. While the emergence of CRISPR-Cas9 technology has revolutionized gene editing, the use of individual guide RNAs limits the efficiency and application of this technology in functional genetics research. Multiplexed genome editing significantly enhances the efficiency and scope of gene editing. Herein, we describe an efficient multiplexed genome editing strategy to generate zebrafish mutants. Following behavioural tests and histological examination, we identified one new candidate gene (

Indexed as

CRISPR-Cas9hearing lossmultiplexed genome editingtmem183azebrafish

Identifiers

PMID35202345
PMCPMC8879510
OpenAlexW4213022438

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.