Evidence map›Paper›PMID 35201421›Full record

ArticleCancer chemotherapy and pharmacology2022

Disulfiram/copper induces antitumor activity against gastric cancer cells in vitro and in vivo by inhibiting S6K1 and c-Myc.

Cheng Du, Xin Guan, Yao Liu, Zhuxuan Xu, Xiaowei Du, Baolei Li, Meiling Wang, Zhendong Zheng

Abstract read
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In one paragraph

Article in Cancer chemotherapy and pharmacology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 34 citations in OpenAlex.

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  15. Drug repurposing for cancer therapy.Signal transduction and targeted therapy · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Cheng Du *Department of Oncology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenyang, 110840, People's Republic of China.ORCID 0000-0003-0213-9199
Xin Guan *Department of Oncology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenyang, 110840, People's Republic of China.
Yao Liu *Department of Oncology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenyang, 110840, People's Republic of China.
Zhuxuan XuDepartment of Oncology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenyang, 110840, People's Republic of China.
Xiaowei DuDepartment of Oncology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenyang, 110840, People's Republic of China.
Baolei LiDepartment of Oncology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenyang, 110840, People's Republic of China.
Meiling WangDepartment of Oncology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenyang, 110840, People's Republic of China.
Zhendong ZhengDepartment of Oncology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenyang, 110840, People's Republic of China. gcp_zzd@sina.com.
General Hospital of Shenyang Military Region · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeDisulfiram (DSF) is an approved drug for the treatment of alcohol dependence. Accumulating evidence indicates that DSF, alone or in combination with copper (Cu), possesses strong antitumor activity in various malignancies. This study investigated the effects of DSF on gastric cancer (GC) and the potential mechanisms involved.

methodsGC cell proliferation and apoptosis upon treatment with DSF with or without copper were analyzed using CCK-8 assay, colony formation assay, and flow cytometry. Glucose metabolism was investigated using glucose consumption and lactate production assays. The expression of caspase-3, Bcl-2, LC-3, P62, S6K1, c-Myc, GLUT1, PKM2, and LDHA was analyzed using western blot assay. In vivo nude mice studies were performed to verify the findings from in vitro analyses.

resultsOur study showed that DSF was highly toxic to GC cells in a Cu-dependent manner. Nontoxic concentrations of Cu enhanced the inhibitory effects of DSF on cell viability and colony formation. DSF also induced apoptotic and autophagic cell death in the presence of Cu. In addition, DSF/Cu inhibited glycolysis and xenograft growth of GC cells by suppressing the expression of S6K1, c-Myc, and their downstream molecules, including GLUT1, PKM2, and LDHA.

conclusionOur study demonstrated that DSF/Cu exerted antitumor activity against GC cells both in vitro and in vivo. DSF/Cu may represent a promising therapeutic strategy for the treatment of GC.

Indexed as

DisulfiramStomach NeoplasmsAnimalsCell Line, TumorCopperGlucose Transporter Type 1HumansMiceMice, NudeProto-Oncogene Proteins c-mycRibosomal Protein S6 Kinases, 70-kDaXenograft Model Antitumor AssaysCopperDisulfiramGlucose Transporter Type 1MYC protein, humanProto-Oncogene Proteins c-mycribosomal protein S6 kinase, 70kD, polypeptide 1Ribosomal Protein S6 Kinases, 70-kDac-MycCopperCytotoxicityDisulfiramGastric cancerGlycolysis

Identifiers

PMID35201421
OpenAlexW4214685118

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.