ArticleBiosensors2022
A Recombinase-Based Genetic Circuit for Heavy Metal Monitoring.
Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The trial behind it
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Advances and perspectives in genetically encoded biosensors for biomarker detection and theranostic circuits.Synthetic and systems biotechnology · 2027Review
- Living Microbial Drugs.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Tunable low-rate genomic recombination with Cre-Applied and environmental microbiology · 2026Article
- Beyond carbon capture: bioengineering cyanobacteria as solar-driven platforms for remediation of recalcitrant human-generated waste.Frontiers in plant science · 2026Review
- Wireless in-body sensing through genetically engineered bacteria.Nature communications · 2025Article
- Characterization of recombinase activity across cellular growth phases.Scientific reports · 2025Article
- Review
- Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts.Nature communications · 2024Article
- A Genetic Circuit Design for Targeted Viral RNA Degradation.Bioengineering (Basel, Switzerland) · 2023Article
- A review of biosensor for environmental monitoring: principle, application, and corresponding achievement of sustainable development goals.Bioengineered · 2023Review
- Special Issue "Synthetic Biology for Biosensing in Health and Environmental Applications".Biosensors · 2023Article
- Next generation synthetic memory via intercepting recombinase function.Nature communications · 2023Article
- Construction of a bacteriophage-derived recombinase system in Bacillus licheniformis for gene deletion.AMB Express · 2023Article
- Article
- Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Rapid progress in the genetic circuit design enabled whole-cell biosensors (WCBs) to become prominent in detecting an extensive range of analytes with promise in many fields, from medical diagnostics to environmental toxicity assessment. However, several drawbacks, such as high background signal or low precision, limit WCBs to transfer from proof-of-concept studies to real-world applications, particularly for heavy metal toxicity monitoring. For an alternative WCB module design, we utilized Bxb1 recombinase that provides tight control as a switch to increase dose-response behavior concerning leakiness. The modularity of Bxb1 recombinase recognition elements allowed us to combine an engineered semi-specific heat shock response (HSR) promoter, sensitive to stress conditions including toxic ions such as cadmium, with cadmium resistance regulatory elements; a cadmium-responsive transcription factor and its cognitive promoter. We optimized the conditions for the recombinase-based cadmium biosensor to obtain increased fold change and shorter response time. This system can be expanded for various heavy metals to make an all-in-one type of WCB, even using semi-specific parts of a sensing system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.