Evidence map›Paper›PMID 35200336›Full record

ArticleBiosensors2022

Rapid Detection of Virus Nucleic Acid via Isothermal Amplification on Plasmonic Enhanced Digitizing Biosensor.

Shih-Chung Wei, Chia-Chen Chang, Tsung-Liang Chuang, Kung-Bin Sung, Chii-Wann Lin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shih-Chung WeiInstitute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.ORCID 0000-0003-0613-6577
Chia-Chen ChangDepartment of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan 33302, Taiwan.ORCID 0000-0001-5466-4724
Tsung-Liang ChuangInstitute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Kung-Bin SungInstitute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.
Chii-Wann LinInstitute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.

Funding

Ministry of Science and Technology of Taiwan 102-2218-E-002-014-MY3
6 · The paper itself

Abstract

Rapid detection for infectious diseases is highly demanded in diagnosis and infection prevention. In this work, we introduced a plasmonic enhanced digitizing biosensor for the rapid detection of nucleic acids. The sensor successfully achieved the detection of loop-mediated isothermal amplification for the hepatitis virus in this work. The sensor comprised a nanodisc array and Bst polymerases conjugated on the rough surface of a nanodisc. The rough surface of the nanodisc provided plasmonic hot spots to enhance the fluorescence signal. The virus DNA was detected by conducting a modified loop-mediated isothermal amplification with fluorescence resonance energy transfer reporter conjugated primers on the sensor. The modified isothermal amplification improved the signal contrast and detection time compared to the original assay. By integrating the modified amplification assay and plasmonic enhancement sensor, we achieved rapid detection of the hepatitis virus. Nucleic acid with a concentration of 10

Indexed as

Biosensing TechniquesNucleic AcidsDNA PrimersFluorescence Resonance Energy TransferNucleic Acid Amplification TechniquesSensitivity and SpecificityDNA PrimersNucleic AcidsLAMPnanoarrayplasmonic

Identifiers

PMID35200336
PMCPMC8869753

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.