Evidence map›Paper›PMID 35200326›Full record

ArticleBiosensors2022

Magneto-Immunoassay for the Detection and Quantification of Human Growth Hormone.

Almira Ramanaviciene, Anton Popov, Ema Baliunaite, Benediktas Brasiunas, Asta Kausaite-Minkstimiene, Ugur Tamer, Gailute Kirdaite, Eiva Bernotiene, Ali Mobasheri

Open access · goldAbstract read
In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 6 countries.

Almira RamanavicieneDepartment of Immunology, State Research Institute Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania.ORCID 0000-0001-5864-0359
Anton PopovDepartment of Immunology, State Research Institute Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania.ORCID 0000-0002-9867-1931
Ema BaliunaiteNanotechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Institute of Chemistry, Vilnius University, LT-03225 Vilnius, Lithuania.ORCID 0000-0003-1955-0793
Benediktas BrasiunasNanotechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Institute of Chemistry, Vilnius University, LT-03225 Vilnius, Lithuania.
Asta Kausaite-MinkstimieneDepartment of Immunology, State Research Institute Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania.
Ugur TamerDepartment of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Ankara TR-06330, Turkey.
Gailute KirdaiteDepartment of Experimental, Preventive and Clinical Medicine, State Research Institute Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania.
Eiva BernotieneDepartment of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania.
Ali MobasheriDepartment of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania.ORCID 0000-0001-6261-1286
Vilnius University · LTGazi University · TRState Research Institute Centre for Innovative Medicine · LTUniversity of Liège · BEVilnius Gediminas Technical University · LT

Funding

European Regional Development Fund through the Research Council of Lithuania according to the Programme Attracting Foreign Researchers for Research Implementation 01.2.2-LMT-K-718-02-0022.
6 · The paper itself

Abstract

Physiological and endocrine maintenance of a normal human growth hormone (hGH) concentration is crucial for growth, development, and a number of essential biological processes. In this study, we describe the preparation and characterization of magnetic nanoparticles coated with a gold shell (MNPs-Au). The optimal surface concentration of monoclonal anti-hGH antibodies (m-anti-hGH) on magnetic nanoparticles, as well as conditions that decrease non-specific interactions during the magneto-immunoassay, were elaborated. After the selective recognition, separation, and pre-concentration of hGH by MNPs-Au/m-anti-hGH and the hGH interaction with specific polyclonal biotin-labeled antibodies (p-anti-hHG-B) and streptavidin modified horseradish peroxidase (S-HRP), the MNPs-Au/m-anti-hGH/hGH/p-anti-hGH-B/S-HRP immunoconjugate was formed. The concentration of hGH was determined after the addition of 3,3',5,5'-tetramethylbenzidine and hydrogen peroxide substrate solution for HRP; the absorbance at 450 nm was registered after the addition of STOP solution. The developed sandwich-type colorimetric magneto-immunoassay is characterized by a clinically relevant linear range (from 0.1 to 5.0 nmol L

Indexed as

Human Growth HormoneColorimetryGoldHorseradish PeroxidaseHumansImmunoassayGoldHorseradish PeroxidaseHuman Growth Hormonegold shell magnetic nanoparticleshuman growth hormonesandwich-type colorimetric magneto-immunoassay

Identifiers

PMID35200326
PMCPMC8869458
OpenAlexW4210257033

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.