ArticleeLife2022
In vivo fluorescence lifetime imaging of macrophage intracellular metabolism during wound responses in zebrafish.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Illuminating Immunity: A Systematic Review of Immune Cell Autofluorescence.Journal of biophotonics · 2025Pooled it
- Deletion ofBiology · 2026Article
- Optical, Tomographic, and Mass Spectrometry Imaging Methods for Burn Wounds: Capabilities, Limitations, and Clinical Potential.Biomedicines · 2026Review
- Prediction of Patients' Response to Immune Checkpoint Inhibitors Using Fluorescence Lifetime Imaging of Lymphocytes.Biomedicines · 2026Article
- Real-time, high-resolution metabolic characterization of live bacteria using label-free optical metabolic imaging.NPJ biofilms and microbiomes · 2026Article
- Divergent role of CD8 T cells with distinct metabolic phenotypes during curative radio-immunotherapy in hot versus cold tumors.bioRxiv : the preprint server for biology · 2026Article
- Assessing Intracellular Metabolism of Immune Cells In Situ in Live Zebrafish Larvae by Autofluorescence Lifetime Imaging Microscopy of NAD(P)H and FAD.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Mechanisms and therapeutic insights from zebrafish models of wound healing.Frontiers in cell and developmental biology · 2026Review
- Perspectives on label-free microscopy of heterogeneous and dynamic biological systems.Journal of biomedical optics · 2025Article
- Engineering macrophage responses through 3D scaffold microarchitecture.Materials today. Bio · 2025Article
- Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue.Communications biology · 2025Article
- Multispectral autofluorescence for label free classification of immune cell type and activation/polarization status.Scandinavian journal of immunology · 2025Article
- Metabolic rewiring in skin epidermis drives tolerance to oncogenic mutations.Nature cell biology · 2025Article
- Consensus guidelines for cellular label-free optical metabolic imaging: ensuring accuracy and reproducibility in metabolic profiling.Journal of biomedical optics · 2025Article
- Simultaneous Application of Methylene Blue and Chlorin e6 Photosensitizers: Investigation on a Cell Culture.Sovremennye tekhnologii v meditsine · 2025Article
- Unravelling approaches to study macrophages: from classical to novel biophysical methodologies.PeerJ · 2025Review
- Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems.Frontiers in immunology · 2025Article
- Autofluorescence lifetime imaging classifies human B and NK cell activation state.Frontiers in bioengineering and biotechnology · 2025Article
- Deep and dynamic metabolic and structural imaging in living tissues.Science advances · 2024Article
- Endogenous mitochondrial NAD(P)H fluorescence can predict lifespan.Communications biology · 2024Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
The function of macrophages in vitro is linked to their metabolic rewiring. However, macrophage metabolism remains poorly characterized in situ. Here, we used two-photon intensity and lifetime imaging of autofluorescent metabolic coenzymes, nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD), to assess the metabolism of macrophages in the wound microenvironment. Inhibiting glycolysis reduced NAD(P)H mean lifetime and made the intracellular redox state of macrophages more oxidized, as indicated by reduced optical redox ratio. We found that TNFα+ macrophages had lower NAD(P)H mean lifetime and were more oxidized compared to TNFα- macrophages. Both infection and thermal injury induced a macrophage population with a more oxidized redox state in wounded tissues. Kinetic analysis detected temporal changes in the optical redox ratio during tissue repair, revealing a shift toward a more reduced redox state over time. Metformin reduced TNFα+ wound macrophages, made intracellular redox state more reduced and improved tissue repair. By contrast, depletion of STAT6 increased TNFα+ wound macrophages, made redox state more oxidized and impaired regeneration. Our findings suggest that autofluorescence of NAD(P)H and FAD is sensitive to dynamic changes in intracellular metabolism in tissues and can be used to probe the temporal and spatial regulation of macrophage metabolism during tissue damage and repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.