ArticleSTAR protocols2022
Isolation and feeder-free primary culture of four cell types from a single human skin sample.
Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- CRISPR/Cas9-Mediated Correction of the SLC25A38 Mutation in a Patient With Sideroblastic Anemia.The journal of gene medicine · 2026Article
- Dermal Templates Support Epidermal Regeneration and Maturation Regardless of Cell Donor Age.Tissue engineering. Part A · 2026Article
- IFN-κ directs antiviral immunity in human skin.bioRxiv : the preprint server for biology · 2026Article
- High-yield isolation and characterization of microvascular niche cells from human full-thickness skin biopsies.Scientific reports · 2026Article
- In Vitro Skin Models as Non-Animal Methods for Dermal Drug Development and Safety Assessment.Pharmaceutics · 2025Review
- Effects of peptides derived from active sites of visfatin on wound healing.Scientific reports · 2025Article
- Donor Variability Alters the Characteristics of Human Brain Microvascular Endothelial Cells.Current issues in molecular biology · 2025Article
- Article
- Comparison of Different Keratinocyte Cell Line Models for Analysis of NLRP1 Inflammasome Activation.Biomolecules · 2024Article
- Establishing Primary and Stable Cell Lines from Frozen Wing Biopsies for Cellular, Physiological, and Genetic Studies in Bats.Current protocols · 2024Article
- Regulation of MYC by CARD14 in human epithelium is a determinant of epidermal homeostasis and disease.Cell reports · 2024Article
- Establishing Primary and Stable Cell Lines from Frozen Wing Biopsies for Cellular, Physiological, and Genetic Studies in Bats.bioRxiv : the preprint server for biology · 2024Article
- Cold dispase digestion of murine lungs improves recovery and culture of airway epithelial cells.PloS one · 2024Article
- Efficient protocol for isolating human fibroblast from primary skin cell cultures: application to keloid, hypertrophic scar, and normal skin biopsies.Biology methods & protocols · 2024Article
- Comparison of Two Human Skin Cell Isolation Protocols and Their Influence on Keratinocyte and Fibroblast Culture.International journal of molecular sciences · 2023Article
- Glycolipid Biosurfactants in Skincare Applications: Challenges and Recommendations for Future Exploitation.Molecules (Basel, Switzerland) · 2023Review
- Myofibroblasts Are Not Characteristic Features of Keloid Lesions.Plastic and reconstructive surgery. Global open · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four types of primary cells-dermal fibroblasts, dermal microvascular endothelial cells, epidermal keratinocytes, and epidermal melanocytes-can be isolated simultaneously from a single human skin sample, without the use of xenogeneic murine feeder cells. This protocol describes the procedures for isolation of these cells from adult full-thickness skin obtained from surgical discard tissue. The cells isolated using this protocol contain stem cell populations and are competent to form functional skin tissue in three-dimensional reconstructed skin models. For complete details on the use and execution of this profile, please refer to Supp et al. (2002), Boyce et al. (2015), Boyce et al. (2017a), Boyce et al. (2017b), and Supp et al. (2019).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.