Evidence map›Paper›PMID 35198129›Full record

ArticleComputational and structural biotechnology journal2022

Structural and biochemical characterization of the relaxosome auxiliary proteins encoded on the

Isidro Crespo, Nerea Bernardo, Anna Cuppari, Barbara M Calisto, Jorge Val-Calvo, Andrés Miguel-Arribas, Wilfried J J Meijer, Xavi Carpena, Fernando Gil-Ortiz, Marc Malfois and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Computational and structural biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Isidro CrespoALBA Synchrotron Light Source, Carrer de la Llum 2-26, Cerdanyola del Vallès, 08290 Barcelona, Spain.
Nerea BernardoALBA Synchrotron Light Source, Carrer de la Llum 2-26, Cerdanyola del Vallès, 08290 Barcelona, Spain.
Anna CuppariALBA Synchrotron Light Source, Carrer de la Llum 2-26, Cerdanyola del Vallès, 08290 Barcelona, Spain.
Barbara M CalistoALBA Synchrotron Light Source, Carrer de la Llum 2-26, Cerdanyola del Vallès, 08290 Barcelona, Spain.
Jorge Val-CalvoCentro de Biología Molecular "Severo Ochoa" (CSIC-UAM), C. Nicolás Cabrera 1, Universidad Autónoma, Canto Blanco, 28049 Madrid, Spain.
Andrés Miguel-ArribasCentro de Biología Molecular "Severo Ochoa" (CSIC-UAM), C. Nicolás Cabrera 1, Universidad Autónoma, Canto Blanco, 28049 Madrid, Spain.
Wilfried J J MeijerCentro de Biología Molecular "Severo Ochoa" (CSIC-UAM), C. Nicolás Cabrera 1, Universidad Autónoma, Canto Blanco, 28049 Madrid, Spain.
Xavi CarpenaALBA Synchrotron Light Source, Carrer de la Llum 2-26, Cerdanyola del Vallès, 08290 Barcelona, Spain.
Fernando Gil-OrtizALBA Synchrotron Light Source, Carrer de la Llum 2-26, Cerdanyola del Vallès, 08290 Barcelona, Spain.
Marc MalfoisALBA Synchrotron Light Source, Carrer de la Llum 2-26, Cerdanyola del Vallès, 08290 Barcelona, Spain.
D Roeland BoerALBA Synchrotron Light Source, Carrer de la Llum 2-26, Cerdanyola del Vallès, 08290 Barcelona, Spain.
ALBA Synchrotron (Spain) · ESCentro de Biología Molecular Severo Ochoa · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial conjugation is an important route for horizontal gene transfer. The initial step in this process involves a macromolecular protein-DNA complex called the relaxosome, which in plasmids consists of the origin of transfer (

Indexed as

Antibiotic resistanceAUC, Analytical UltracentrifugationAuxiliary proteinBacterial conjugationDNA binding proteinEDTA, Ethylenediaminetetraacetic acidEM, Electron MicoscopyFirmicutesHGT, Horizontal Gene TransferHorizontal gene transferIPTG, Isopropyl β-D-1-thiogalactopyranosideITC, Isothermal titration calorimetryMGE, Mobile Genetic ElementoriT, Origin of TransferPMSF, phenylmethylsulfonyl fluorideRelaxosomeRHH, Ribbon-Helix-HelixRibbon-Helix-HelixSAXS, Small-angle X-ray scatteringSDS-PAGE, sodium dodecyl sulphate–polyacrylamide gel electrophoresisSEC, Size Exclusion ChromatographyStructural biologyT4SS, Type IV secretion system

Identifiers

PMID35198129
PMCPMC8829557
OpenAlexW4206739887

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.