Evidence map›Paper›PMID 35198102›Full record

ArticleOncotarget2022

A liquid fraction of extracellular matrix inhibits glioma cell viability

Mark H Murdock, George S Hussey, Jordan T Chang, Ryan C Hill, David G Nascari, Aparna V Rao, Kirk C Hansen, Lesley M Foley, T Kevin Hitchens, Nduka M Amankulor and 1 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Mark H MurdockDepartment of Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
George S HusseyDepartment of Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
Jordan T ChangMcGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Ryan C HillDepartment of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, CO, USA.
David G NascariDepartment of Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
Aparna V RaoDepartment of Neurobiology, University of Pittsburgh, Pittsburgh, PA, USA.
Kirk C HansenDepartment of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, CO, USA.
Lesley M FoleyAnimal Imaging Center, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
T Kevin HitchensAnimal Imaging Center, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Nduka M AmankulorDepartment of Neurological Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
Stephen F BadylakDepartment of Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
University of Pittsburgh · USMcGowan Institute for Regenerative Medicine · USUniversity of Colorado Denver · US

Funding

VECTOR CORE FACILITYP30CA047904 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI JOHN C. BYRD · 1988 to 2026
$158.0M
NRSA Training CoreTL1TR001858 · NCATS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI RADOMSKI, THOMAS, RUBIO, DORIS M · 2016 to 2025
$11.1M
Quadrupole-Orbitrap Mass SpectrometerS10OD021641 · OD · UNIVERSITY OF COLORADO DENVER · PI HANSEN, KIRK C · 2017 to 2017
$600k
NCATS NIH HHS TL1 TR001858NCI NIH HHS P30 CA047904NIH HHS S10 OD021641
6 · The paper itself

Abstract

Suppressive effects of extracellular matrix (ECM) upon various cancers have been reported. Glioblastoma multiforme has poor prognosis and new therapies are desired. This work investigated the effects of a saline-soluble fraction of urinary bladder ECM (ECM-SF) upon glioma cells. Viability at 24 hours in 1, 5, or 10 mg/mL ECM-SF-spiked media was evaluated in primary glioma cells (0319, 1015, 1119), glioma cell lines (A172, T98G, U87MG, C6), and brain cell lines (HCN-2, HMC3). Viability universally decreased at 5 and 10 mg/mL with U87MG, HCN-2, and HCM3 being least sensitive. Apoptosis in 0319 and 1119 cells was confirmed via NucView 488. Bi-weekly intravenous injection of ECM-SF (120 mg/kg) for 10 weeks in Sprague-Dawley rats did not affect weight, temperature, complete blood count, or multi-organ histology (

Indexed as

Brain NeoplasmsGlioblastomaGliomaAnimalsCell Line, TumorCell SurvivalExtracellular MatrixRatsRats, Sprague-DawleyRats, Wistarbrain cancerdynamic reciprocityextracellular matrixglioma treatmenttissue organization field theory

Identifiers

PMID35198102
PMCPMC8860176
OpenAlexW4213037652

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.