ArticlePloS one2022
Decay and damage of therapeutic phage OMKO1 by environmental stressors.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 0 citations in OpenAlex.
- Article
- Effect of human urinary microenvironment and fluid flow on antibiotic and phage therapy efficacy against uropathogenic Escherichia coli.Nature communications · 2026Article
- Host physiology-dependent lysis timing shapes bacteriophage competition under nutrient fluctuations.mSystems · 2026Article
- Lifestyle-dependent evolution and CtrA-mediated lifestyle transitions shape phage resistance in marine Roseobacter.Nature communications · 2026Article
- Rage against the mean: a perspective on measuring fitness of individual phage particles.Npj viruses · 2026Review
- Inactivating conditions of therapeutic mycobacteriophages.Microbiology spectrum · 2026Article
- Can phage-antibiotic combinations overcome uropathogenic Escherichia coli regrowth? evidence from in vitro and in vivo models.Virology journal · 2026Article
- Inactivating conditions of therapeutic mycobacteriophages.bioRxiv : the preprint server for biology · 2025Article
- Frenemy: adaptive temperate phage_SAP_1432 supportsMicrobiology spectrum · 2025Article
- Design, optimization, and inference of biphasic decay of infectious virus particles.Journal of theoretical biology · 2025Article
- Design, optimization, and inference of biphasic decay of infectious virus particles.bioRxiv : the preprint server for biology · 2024Article
- More evolvable bacteriophages better suppress their host.Evolutionary applications · 2024Article
- Warming alters life-history traits and competition in a phage community.Applied and environmental microbiology · 2024Article
- Article
- Stability study in selected conditions and biofilm-reducing activity of phages active against drug-resistant Acinetobacter baumannii.Scientific reports · 2024Article
- Antimicrobial resistance expansion in pathogens: a review of current mitigation strategies and advances towards innovative therapy.JAC-antimicrobial resistance · 2023Review
- Article
- Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY.Cell reports · 2023Article
- How to Tackle Bacteriophages: The Review of Approaches with Mechanistic Insight.International journal of molecular sciences · 2023Review
- Bacteriophage Effectively Rescues Pneumonia Caused by Prevalent Multidrug-ResistantMicrobiology spectrum · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibiotic resistant bacterial pathogens are increasingly prevalent, driving the need for alternative approaches to chemical antibiotics when treating infections. One such approach is bacteriophage therapy: the use of bacteria-specific viruses that lyse (kill) their host cells. Just as the effect of environmental conditions (e.g. elevated temperature) on antibiotic efficacy is well-studied, the effect of environmental stressors on the potency of phage therapy candidates demands examination. Therapeutic phage OMKO1 infects and kills the opportunistic human pathogen Pseudomonas aeruginosa. Here, we used phage OMKO1 as a model to test how environmental stressors can lead to damage and decay of virus particles. We assessed the effects of elevated temperatures, saline concentrations, and urea concentrations. We observed that OMKO1 particles were highly tolerant to different saline concentrations, but decayed more rapidly at elevated temperatures and under high concentrations of urea. Additionally, we found that exposure to elevated temperature reduced the ability of surviving phage particles to suppress the growth of P. aeruginosa, suggesting a temperature-induced damage. Our findings demonstrate that OMKO1 is highly tolerant to a range of conditions that could be experienced inside and outside the human body, while also showing the need for careful characterization of therapeutic phages to ensure that environmental exposure does not compromise their expected potency, dosing, and pharmacokinetics.
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