Evidence map›Paper›PMID 35196166›Full record

ArticleAmerican journal of physiology. Cell physiology2022

Serially passaged, conditionally reprogrammed nasal epithelial cells as a model to study epithelial functions and SARS-CoV-2 infection.

Hanna Schmidt, Lara Guthjahr, Alexander Sauter, Fabian Zech, Rayhane Nchioua, Steffen Stenger, Manfred Frick, Frank Kirchhoff, Paul Dietl, Oliver H Wittekindt

Abstract read
In one paragraph

Article in American journal of physiology. Cell physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Hanna SchmidtDepartment of Pediatric and Adolescent Medicine, Ulm University Medical Center, Ulm, Germany.ORCID 0000-0002-6292-030X
Lara GuthjahrInstitute of General Physiology, Ulm University, Ulm, Germany.
Alexander SauterInstitute of General Physiology, Ulm University, Ulm, Germany.
Fabian ZechInstitute of Molecular Virology, Ulm University Medical Center, Ulm, Germany.
Rayhane NchiouaInstitute of Molecular Virology, Ulm University Medical Center, Ulm, Germany.
Steffen StengerInstitute of Medical Microbiology and Hygiene, Ulm University Medical Center, Ulm, Germany.
Manfred FrickInstitute of General Physiology, Ulm University, Ulm, Germany.ORCID 0000-0002-4763-1104
Frank KirchhoffInstitute of Molecular Virology, Ulm University Medical Center, Ulm, Germany.
Paul DietlInstitute of General Physiology, Ulm University, Ulm, Germany.
Oliver H WittekindtInstitute of General Physiology, Ulm University, Ulm, Germany.ORCID 0000-0002-1890-6471

Funding

Deutsche Forschungsgemeinschaft (DFG) 458685876
6 · The paper itself

Abstract

Primary airway epithelial cells (pAECs) cultivated at air-liquid interface (ALI) conditions are widely used as surrogates for human in vivo epithelia. To extend the proliferative capacity and to enable serially passaging of pAECs, conditional reprogramming (cr) has been employed in recent years. However, ALI epithelia derived from cr cells often display functional changes with increasing passages. This highlights the need for thorough validation of the ALI cultures for the respective application. In our study, we evaluated the use of serially passaged cr nasal epithelial cells (crNECs) as a model to study SARS-CoV-2 infection and effects on ion and water transport. NECs were obtained from healthy individuals and cultivated as ALI epithelia derived from

Indexed as

COVID-19Cell DifferentiationCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsHumansInfant, NewbornSARS-CoV-2Cystic Fibrosis Transmembrane Conductance Regulatorairway epithelial cultureconditional reprogrammingnasal epithelial cellsSARS-CoV-2

Identifiers

PMID35196166
PMCPMC8977148

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.