ArticleAngewandte Chemie (International ed. in English)2022
Expressed Protein Selenoester Ligation.
Article in Angewandte Chemie (International ed. in English), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Revealing and Exploiting the Biochemistry ofBiochemistry · 2026Review
- Catalysis of Native Chemical Ligation and Expressed Protein Ligation by Alkylselenols.JACS Au · 2025Article
- Hydrophilic BIPHEPHOS Ligand for Pd-Mediated Cysteine Allylation of Peptides and Proteins in Water.Organic letters · 2025Article
- Site-specifically Phosphorylated Hsp90C-terminal Domain Variants Provide Access to Deciphering the Chaperone Code.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Post-Translational Modifications Control Phase Transitions of Tau.ACS central science · 2024Article
- Post-Translational Modifications Control Phase Transitions of Tau.bioRxiv : the preprint server for biology · 2024Article
- Semisynthesis of A6-A11 lactam insulin.Journal of peptide science : an official publication of the European Peptide Society · 2024Article
- The semisynthesis of nucleolar human selenoprotein H.Chemical science · 2023Article
- HSP27 Inhibitory Activity against Caspase-3 Cleavage and Activation by Caspase-9 Is Enhanced by Chaperone O-GlcNAc ModificationACS chemical biology · 2023Article
- Light-Cleavable Auxiliary for Diselenide-Selenoester Ligations of Peptides and Proteins.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- Cysteine-Selective Modification of Peptides and Proteins via Desulfurative C-C Bond Formation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- Synthesis of Selenoesters via Aldol Condensation and/or Conjugate Reduction and Their Antiviral Activities.ACS omega · 2023Article
- Site-selective photocatalytic functionalization of peptides and proteins at selenocysteine.Nature communications · 2022Article
- Semisynthesis of Homogeneous, Active Granulocyte Colony-Stimulating Factor Glycoforms.Angewandte Chemie (International ed. in English) · 2022Article
- A versatileChemical science · 2022Article
- Expressed Protein Selenoester Ligation.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- Expressed Protein Selenoester Ligation.Angewandte Chemie (International ed. in English) · 2022Article
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11 authors.
Funding
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Abstract
Herein, we describe the development and application of a novel expressed protein selenoester ligation (EPSL) methodology for the one-pot semi-synthesis of modified proteins. EPSL harnesses the rapid kinetics of ligation reactions between modified synthetic selenopeptides and protein aryl selenoesters (generated from expressed intein fusion precursors) followed by in situ chemoselective deselenization to afford target proteins at concentrations that preclude the use of traditional ligation methods. The utility of the EPSL technology is showcased through the efficient semi-synthesis of ubiquitinated polypeptides, lipidated analogues of the membrane-associated GTPase YPT6, and site-specifically phosphorylated variants of the oligomeric chaperone protein Hsp27 at high dilution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.