Evidence map›Paper›PMID 35194841›Full record

ArticleProteins2022

The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.

Brendan J Mahoney, Allen Takayesu, Anqi Zhou, Duilio Cascio, Robert T Clubb

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteins, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Unique Fn3-like biosensor in σProtein science : a publication of the Protein Society · 2024
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Brendan J MahoneyDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.
Allen TakayesuDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.
Anqi ZhouDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.
Duilio CascioUCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, Los Angeles, California, USA.
Robert T ClubbDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.
University of California, Los Angeles · US

Funding

User Training and OutreachP30GM124165 · NIGMS · CORNELL UNIVERSITY · PI Nozomi Ando, J Christopher Fromme · 2018 to 2026
$34.2M
User Training and OutreachP30GM124169 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI PAUL David ADAMS, Gregory L Hura · 2017 to 2026
$28.6M
Cell Surface Protein Anchoring in Gram-Positive BacteriaR01AI052217 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Thompson Clubb · 2002 to 2026
$8.5M
800 MHz NMR Console and Cryoprobe ReplacementS10OD025073 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FEIGON, JULI · 2018 to 2018
$1.1M
A Pixel Array Detector System for Small Angle X-ray ScatteringS10OD018483 · OD · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI CLASSEN, SCOTT · 2014 to 2014
$998k
Bruker Avance III Console and QCI Cryoprobe for a 600 MHz NMR SpectrometerS10OD016336 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CLUBB, ROBERT THOMPSON · 2014 to 2014
$600k
NIAID NIH HHS R01 AI052217NIGMS ALS-ENABLE S10OD018483NIGMS NIH HHS P30 GM124165NIGMS NIH HHS P30 GM124169NIH HHS R01-AI052217NIH HHS S10 OD016336NIH HHS S10OD016336NIH HHS S10 OD018483NIH HHS S10 OD025073NIH HHS S10OD025073
6 · The paper itself

Abstract

Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass. An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes. Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors. Here we show that the RsgI9 anti-σ factor interacts with cellulose via a C-terminal bi-domain unit. A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose. Small-angle X-ray scattering experiments of the intact ectodomain indicate that it adopts a bi-lobed, elongated conformation. In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface. The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.

Indexed as

Acetivibrio thermocellusCellulosomesBacterial ProteinsBiomassCelluloseProlineSigma FactorBacterial ProteinsCelluloseProlineSigma Factoranti-sigma factorbiofuelsbiomasscellulosomescrystallographysmall-angle X-ray scattering

Identifiers

PMID35194841
PMCPMC9177573
OpenAlexW4212901797

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.