Evidence map›Paper›PMID 35194678›Full record

ArticleParasitology research2022

Clinical and molecular diagnosis of Acanthamoeba keratitis in contact lens wearers in southern Brazil reveals the presence of an endosymbiont.

Denise Leal Dos Santos, Veridiana Gomes Virginio, Francisco Kercher Berté, Karina Rodrigues Lorenzatto, Diane Ruschel Marinho, Sergio Kwitko, Claudete Inês Locatelli, Eduarda Correa Freitas, Marilise Brittes Rott

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Article in Parasitology research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.7field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Denise Leal Dos SantosMicrobiology, Immunology and Parasitology Department, Basic Health Sciences Institute, Parasitology Sector, Rio Grande Do Sul Federal University, Sarmento Leite Street, N 500, Porto Alegre, Rio Grande Do Sul, 90050-170, Brazil.
Veridiana Gomes VirginioMicrobiology, Immunology and Parasitology Department, Basic Health Sciences Institute, Parasitology Sector, Rio Grande Do Sul Federal University, Sarmento Leite Street, N 500, Porto Alegre, Rio Grande Do Sul, 90050-170, Brazil.
Francisco Kercher BertéMicrobiology, Immunology and Parasitology Department, Basic Health Sciences Institute, Parasitology Sector, Rio Grande Do Sul Federal University, Sarmento Leite Street, N 500, Porto Alegre, Rio Grande Do Sul, 90050-170, Brazil.
Karina Rodrigues LorenzattoMicrobiology, Immunology and Parasitology Department, Basic Health Sciences Institute, Parasitology Sector, Rio Grande Do Sul Federal University, Sarmento Leite Street, N 500, Porto Alegre, Rio Grande Do Sul, 90050-170, Brazil.
Diane Ruschel MarinhoCornea Department, Ophthalmology Service, Hospital de Clínicas de Porto Alegre Rio Grande Do Sul, Ramiro Barcelos Street, N 2350, Porto Alegre, Rio Grande Do Sul, 90035-903, Brazil.
Sergio KwitkoCornea Department, Ophthalmology Service, Hospital de Clínicas de Porto Alegre Rio Grande Do Sul, Ramiro Barcelos Street, N 2350, Porto Alegre, Rio Grande Do Sul, 90035-903, Brazil.
Claudete Inês LocatelliCornea Department, Ophthalmology Service, Hospital de Clínicas de Porto Alegre Rio Grande Do Sul, Ramiro Barcelos Street, N 2350, Porto Alegre, Rio Grande Do Sul, 90035-903, Brazil.
Eduarda Correa FreitasCornea Department, Ophthalmology Service, Hospital de Clínicas de Porto Alegre Rio Grande Do Sul, Ramiro Barcelos Street, N 2350, Porto Alegre, Rio Grande Do Sul, 90035-903, Brazil.
Marilise Brittes RottMicrobiology, Immunology and Parasitology Department, Basic Health Sciences Institute, Parasitology Sector, Rio Grande Do Sul Federal University, Sarmento Leite Street, N 500, Porto Alegre, Rio Grande Do Sul, 90050-170, Brazil. marilise.rott@ufrgs.br.ORCID http://orcid.org/0000-0002-5227-4594
Universidade Federal do Rio Grande do Sul · BRHospital de Clínicas de Porto Alegre · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acanthamoeba keratitis (AK) is an infection that is mostly observed in contact lens wearers. It is often misdiagnosed causing delays in the administration of the correct treatment. The aim of this study was to report the outcome of clinical and molecular diagnosis of AK cases during the summer of 2019 in the southern region of Brazil. Three suspected cases of AK were discovered after an ophthalmic examination at a public hospital in the city of Porto Alegre. These cases were then confirmed through laboratory diagnosis (cell culture and molecular analysis by PCR and sequencing). In each of the three clinical sample cell cultures of corneal scraping and molecular analysis confirmed the presence of Acanthamoeba spp., all belonging to the morphological group II and to the genotype T4, which is the most common genotype associated with AK. In addition, Acanthamoeba spp. isolated from one of the clinical samples was found to harbor the Candidatus Paracaedibacter acanthamoeba, a bacterial endosymbiont. The presence of Ca. Paracaedibacter acanthamoeba in clinical isolates requires further research to reveal its possible role in the pathogenicity of Acanthamoeba infections.

Indexed as

AcanthamoebaAcanthamoeba KeratitisAmebiasisContact LensesBrazilGenotypeHumansAcanthamoeba keratitisCandidatus Paracaedibacter acanthamoebaeContact lensesEndosymbiontGenotype T4

Identifiers

PMID35194678
OpenAlexW4213439008

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.