Evidence map›Paper›PMID 35193938›Full record

ArticleGenome research2022

Low-input RNase footprinting for simultaneous quantification of cytosolic and mitochondrial translation.

Qianru Li, Haiwang Yang, Emily K Stroup, Hongbin Wang, Zhe Ji

Erratum issuedOpen access · bronzeAbstract read
In one paragraph

Article in Genome research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 39 citations in OpenAlex.

  1. Article
  2. An upstream open reading frame represses translation of the neuronal potassium channel KCNQ2.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Advances in ribosome profiling technologies.Biochemical Society transactions · 2025
    Review
  12. Article
  13. Review
  14. Article
  15. Noncanonical microprotein regulation of immunity.Molecular therapy : the journal of the American Society of Gene Therapy · 2024
    Review
  16. Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Qianru Li *Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
Haiwang Yang *Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
Emily K StroupDepartment of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
Hongbin WangDepartment of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
Zhe JiDepartment of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
Northwestern University · US

Funding

Characterizing functional translation in putative 'noncoding' regions of a genome: Admin SupplR35GM138192 · NIGMS · NORTHWESTERN UNIVERSITY AT CHICAGO · PI JI, ZHE · 2020 to 2024
$2.1M
An ultra-low-input RNase footprinting assay to quantify cytosolic and mitochondrial translation simultaneouslyR01HL161389 · NHLBI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI JI, ZHE · 2022 to 2025
$1.9M
Predoctoral Training Program in Biomedical Data Driven Discovery (BD3)T32LM012203 · NLM · NORTHWESTERN UNIVERSITY AT CHICAGO · PI KLABJAN, DIEGO, STARREN, JUSTIN B. · 2015 to 2019
$1.1M
Dissect regulation of RNA translation in human cancersR00CA207865 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI JI, ZHE · 2018 to 2020
$705k
NCI NIH HHS R00 CA207865NHLBI NIH HHS R01 HL161389NIGMS NIH HHS R35 GM138192NLM NIH HHS T32 LM012203
6 · The paper itself

Abstract

We describe a low-input RNase footprinting approach for the rapid quantification of ribosome-protected fragments with as few as 1000 cultured cells. The assay uses a simplified procedure to selectively capture ribosome footprints based on optimized RNase digestion. It simultaneously maps cytosolic and mitochondrial translation with single-nucleotide resolution. We applied it to reveal selective functions of the elongation factor TUFM in mitochondrial translation, as well as synchronized repression of cytosolic translation after

Indexed as

Protein BiosynthesisRibonucleasesOpen Reading FramesRibosomesRNA, MessengerRibonucleasesRNA, Messenger

Identifiers

PMID35193938
PMCPMC8896460
OpenAlexW4213456750

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.