Evidence map›Paper›PMID 35193085›Full record

ArticleClinics (Sao Paulo, Brazil)2022

Effects of ganglioside GM1 and erythropoietin on spinal cord injury in mice: Functional and immunohistochemical assessments.

Alessandro Gonzalez Torelli, Alexandre Fogaça Cristante, Tarcísio Eloy Pessoa de Barros-Filho, Gustavo Bispo Dos Santos, Beatriz Cintra Morena, Felipe Fernandes Correia, Vera Paschon

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinics (Sao Paulo, Brazil), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Alessandro Gonzalez TorelliDivisão de Cirurgia de Coluna Vertebral, Instituto de Ortopedia e Traumatologia (IOT), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), São Paulo, SP, Brazil. Electronic address: alessandro.torelli@hc.fm.usp.br.
Alexandre Fogaça CristanteDivisão de Cirurgia de Coluna Vertebral, Instituto de Ortopedia e Traumatologia (IOT), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), São Paulo, SP, Brazil.
Tarcísio Eloy Pessoa de Barros-FilhoDivisão de Cirurgia de Coluna Vertebral, Instituto de Ortopedia e Traumatologia (IOT), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), São Paulo, SP, Brazil.
Gustavo Bispo Dos SantosLaboratório de Investigação Médica (LIM 41), Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, SP, Brazil.
Beatriz Cintra MorenaLaboratório de Neurogenética, Universidade Federal do ABC, Santo Andre, SP, Brazil.
Felipe Fernandes CorreiaLaboratório de Neurogenética, Universidade Federal do ABC, Santo Andre, SP, Brazil.
Vera PaschonLaboratório de Neurogenética, Universidade Federal do ABC, Santo Andre, SP, Brazil.
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo · BRUniversidade Federal do ABC · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo evaluate the functional and immunohistochemical effects of ganglioside GM1 and erythropoietin following experimental spinal cord injury.

methodsThirty-two male BALB/c mice were subjected to experimental spinal cord injury using the NYU Impactor device and were randomly divided into the following groups: GM1 group, receiving standard ganglioside GM1 (30 mg/kg); erythropoietin group, receiving erythropoietin (1000 IU/kg); combination group, receiving both drugs; and control group, receiving saline (0.9%). Animals were evaluated according to the Basso Mouse Scale (BMS) and Hindlimb Mouse Function Score (MFS). After euthanasia, the immunohistochemistry of the medullary tissue of mice was analyzed. All animals received intraperitoneal treatment.

resultsThe GM1 group had higher BMS and MFS scores at the end of the experiment when compared to all other groups. The combination group had higher BMS and MFS scores than the erythropoietin and control groups. The erythropoietin group had higher BMS and MFS scores than the control group. Immunohistochemical tissue analysis showed a significant difference among groups. There was a significant increase in myelinated axons and in the myelinated axon length in the erythropoietin group when compared to the other intervention groups (p < 0.01).

conclusionsErythropoietin and GM1 have therapeutic effects on axonal regeneration in mice subjected to experimental spinal cord injury, and administration of GM1 alone had the highest scores on the BMS and MFS scales.

Indexed as

ErythropoietinSpinal Cord InjuriesAnimalsDisease Models, AnimalEpoetin AlfaG(M1) GangliosideInjections, IntraperitonealMaleMiceSpinal CordEpoetin AlfaErythropoietinG(M1) GangliosideErythropoietinGM1 gangliosideMiceSpinal cordSpinal cord compression

Identifiers

PMID35193085
PMCPMC8903807
OpenAlexW4212814012

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.