Evidence map›Paper›PMID 35192189›Full record

ArticleJournal of physiology and biochemistry2022

Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention.

Shasha Liu, Jingjing Da, Jiali Yu, Rong Dong, Jing Yuan, Fuxun Yu, Yan Zha

Abstract read
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In one paragraph

Article in Journal of physiology and biochemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. [WD40 repeat-containing protein 6 aggravates diabetic kidney disease by promoting lipid accumulation in kidney].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Shasha LiuSchool of Medicine, Guizhou University, Guiyang, 550025, Guizhou, China.
Jingjing DaSchool of Medicine, Guizhou University, Guiyang, 550025, Guizhou, China.
Jiali YuSchool of Medicine, Guizhou University, Guiyang, 550025, Guizhou, China.
Rong DongDepartment of Nephrology, Guizhou Provincial People's Hospital, 83, Zhongshan Road, Nanming District, Guiyang, 550002, Guizhou, China.
Jing YuanDepartment of Nephrology, Guizhou Provincial People's Hospital, 83, Zhongshan Road, Nanming District, Guiyang, 550002, Guizhou, China.
Fuxun YuNHC Key Laboratory of Pulmonary Immunological Disease, Guizhou Provincial People's Hospital, Guiyang, 550002, Guizhou, China.
Yan ZhaSchool of Medicine, Guizhou University, Guiyang, 550025, Guizhou, China. yanzhagz@126.com.ORCID http://orcid.org/0000-0002-8713-1665
Guizhou University · CNGuizhou Provincial People's Hospital · CN

Funding

Guizhou Science and Technology Department QKHJC2019-1194Guizhou Science and Technology Department QKHJC2020-1Y304Guizhou Science and Technology Department QKHPTRC2018-5636Guizhou Science and Technology Department QKHPTRC2020-2201Guizhou Science and Technology Department QKHZC2019-2801National Natural Science Foundation of China 81760134National Natural Science Foundation of China 81960139
6 · The paper itself

Abstract

Diabetic kidney disease (DKD) is a major health burden closely related to lipid metabolism disorders. Leptin has lipid-lowering efficacy, but the specific mechanism of its local effects on kidney is still unclear. This study aims to investigate the role of ectopic lipid deposition (ELD) in DKD and evaluate the lipid-lowering efficacy of leptin in the palmitic acid (PA)-induced renal tubular epithelial cells (NRK-52E). DKD model was established in Sprague-Dawley (SD) rats by giving single intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) after high-fat diet for 8 weeks. Then, the expression changes of lipid metabolism-related markers were observed. At week 12, the protein expression level of lipid-deposited marker adipose differentiation-related protein (ADRP) was significantly increased. Besides, the lipid synthesis marker sterol regulatory element-binding protein 1c (SREBP 1c) was highly expressed while the expression of insulin-induced gene 1 (Insig-1), a key molecular of inhibiting SREBP 1c, was decreased. Leptin and compound c were incubated with the PA-induced NRK-52E cells to investigate the lipid-lowering effects and whether this effect was mediated by the AMPK/Insig-1/SREBP 1c signaling pathways. mRNA and protein of ADRP and SREBP 1c were reduced after leptin treatment, while Insig-1 and phosphorylated AMP-activated protein kinase (AMPK) were increased. Conversely, inhibition of AMPK phosphorylation by compound c mostly eliminated lipid-lowering efficacy of leptin in PA-induced cells. Collectively, these results suggested that there was ELD of renal tubular epithelial cells in DKD rats. Leptin upregulated the expression level of Insig-1 by activating AMPK to attenuate ELD in PA-induced NRK-52E cells.

Indexed as

Diabetes MellitusDiabetic NephropathiesAMP-Activated Protein KinasesAnimalsLeptinLipid MetabolismPalmitic AcidRatsRats, Sprague-DawleySterol Regulatory Element Binding Protein 1StreptozocinAMP-Activated Protein KinasesLeptinPalmitic AcidSterol Regulatory Element Binding Protein 1StreptozocinAMP-activated protein kinaseDiabetic kidney diseaseEctopic lipid depositionInsulin-induced gene 1Leptin

Identifiers

PMID35192189
OpenAlexW4212781467

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.